CD18 Recombinant Rabbit Monoclonal Antibody [JM71-31]
cat.: ET1703-94
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IP |
| Clonality: | Monoclonal |
| Clone number: | JM71-31 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 85 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human CD18 aa 103-146 / 769. |
| Positive control: | Jurkat cell lysates. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB IP |
1:500-1:1,000 1:10-1:50 |
| Uniprot #: | SwissProt: P05107 Human |
| Alternative names: | 95 subunit beta Beta 2 (antigen CD18 (p95) CD 18 CD18 Cell surface adhesion glycoprotein LFA 1/CR3/P150,959 beta subunit precursor) Cell surface adhesion glycoproteins LFA 1/CR3/p150,95 subunit beta Cell surface adhesion glycoproteins LFA-1/CR3/p150 Complement component 3 receptor 3 and 4 subunit Complement receptor C3 beta subunit Complement receptor C3 subunit beta Integrin beta 2 Integrin beta chain beta 2 Integrin beta-2 (ITGB2) Integrin beta-2 Integrin, beta 2 (complement component 3 receptor 3 and 4 subunit) ITB2_HUMAN ITGB2 LAD LCAMB Leukocyte associated antigens CD18/11A, CD18/11B, CD18/11C Leukocyte cell adhesion molecule CD18 LFA 1 LFA1 Lymphocyte function associated antigen 1 (LFA1) Lymphocyte function associated antigen 1 MAC 1 MAC1 Macrophage antigen 1 (mac1) beta MF17 MFI7 OTTHUMP00000115278 OTTHUMP00000115279 OTTHUMP00000115280 OTTHUMP00000115281 OTTHUMP00000115282 |
Images
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Fig1:
Western blot analysis of CD18 on different lysates with Rabbit anti-CD18 antibody (ET1703-94) at 1/1,000 dilution. Lane 1: Jurkat (Human T lymphocytic leukemia cell) cell lysate Lane 2: Jurkat (Human T lymphocytic leukemia cell) cell lysate (no heat ) Lane 3: U-937 (Human histiocytic lymphoma cell) cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 180 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ET1703-94, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃. Secondary antibody: Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.