Ku 70 Recombinant Rabbit Monoclonal Antibody [JM61-31]
cat.: ET1703-95
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IP, IF-Cell, IF-Tissue, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JM61-31 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 70 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Ku70 aa 521-564 / 609. |
| Positive control: | HeLa cell lysate, Jurkat cell lysate, HeLa, human tonsil tissue. |
| Subcellular location: | Nucleus. Chromosome. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue IHC-P FC IP |
1:500-1:2,000 1:500 1:50-1:200 1:1,000 1:1,000 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P12956 Human |
| Alternative names: | 5'-deoxyribose-5-phosphate lyase Ku70 5'-dRP lyase Ku70 70 kDa subunit of Ku antigen ATP dependent DNA helicase 2 subunit 1 ATP dependent DNA helicase II 70 kDa subunit ATP-dependent DNA helicase 2 subunit 1 ATP-dependent DNA helicase II 70 kDa subunit CTC box binding factor 75 kDa subunit CTC box-binding factor 75 kDa subunit CTC75 CTCBF DNA repair protein XRCC6 G22P1 Ku 70 Ku autoantigen p70 subunit Ku autoantigen, 70kDa Ku p70 Ku70 Ku70 DNA binding component of DNA-dependent proteinkinase complex (thyroid autoantigen 70 kDa Kup70 Lupus Ku autoantigen protein p70 ML8 Thyroid autoantigen 70kD (Ku antigen) Thyroid autoantigen Thyroid lupus autoantigen Thyroid lupus autoantigen p70 Thyroid-lupus autoantigen TLAA X ray repair complementing defective repair in Chinese hamster cells 6 X-ray repair complementing defective repair in Chinese hamster cells 6 X-ray repair cross-complementing protein 6 XRCC 6 Xrcc6 XRCC6_HUMAN |
Images
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Fig1:
Western blot analysis of Ku 70 on different lysates with Rabbit anti-Ku 70 antibody (ET1703-95) at 1/500 dilution. Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 70 kDa Observed band size: 70 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-95) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of HeLa cells labeling Ku 70 with Rabbit anti-Ku 70 antibody (ET1703-95) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Ku 70 antibody (ET1703-95) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-Ku 70 antibody (ET1703-95) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-95) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-Ku 70 antibody (ET1703-95) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-95) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Flow cytometric analysis of HeLa cells labeling Ku 70. Cells were fixed and permeabilized. Then stained with the primary antibody (ET1703-95, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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