Axin2 Recombinant Rabbit Monoclonal Antibody [JM11-30]
cat.: ET1703-96
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JM11-30 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 94 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Axin2 aa 55-93 / 843. |
| Positive control: | HepG2 cell lysate, HeLa cell lysate, SW480 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, human colon cancer tissue, mouse colon tissue. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:2,000 1:50 |
| Uniprot #: | SwissProt: Q9Y2T1 Human | O88566 Mouse | O70240 Rat |
| Alternative names: | Axil Axin like protein Axin-2 Axin-like protein Axin2 AXIN2_HUMAN Axis inhibition protein 2 Conductin DKFZp781B0869 MGC10366 MGC126582 |
Images
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Fig1:
Western blot analysis of Axin2 on different lysates with Rabbit anti-Axin2 antibody (ET1703-96) at 1/500 dilution. Lane 1: HepG2 cell lysate (30 µg/Lane) Lane 2: HeLa cell lysate (30 µg/Lane) Lane 3: SW480 cell lysate (30 µg/Lane) Lane 4: NIH/3T3 cell lysate (30 µg/Lane) Lane 5: PC-12 cell lysate (30 µg/Lane) Predicted band size: 94 kDa Observed band size: 94 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-96) at 1/500 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-Axin2 antibody (ET1703-96) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-96) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-Axin2 antibody (ET1703-96) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-96) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.