FABP4 Recombinant Rabbit Monoclonal Antibody [JM10-99]
cat.: ET1703-98
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-Fr |
| Clonality: | Monoclonal |
| Clone number: | JM10-99 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 15 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human FABP4 aa 60-100. |
| Positive control: | Mouse white adipose tissue lysate, Mouse brown adipose tissue lysate, Mouse kidney tissue lysate, Rat white adipose tissue lysate, Rat brown adipose tissue lysate, Rat kidney tissue lysate, HAEC. |
| Subcellular location: | Nucleus. Cytoplasm. |
| Recommended Dilutions:
WB IF-Cell IHC-Fr |
1:5,000 1:100-1:500 1:100 |
| Uniprot #: | SwissProt: P15090 Human | P04117 Mouse | P70623 Rat |
| Alternative names: | 3T3-L1 lipid-binding protein 422/aP2 A-FABP adipocyte Adipocyte lipid binding protein Adipocyte lipid-binding protein Adipocyte protein AP2 Adipocyte-type fatty acid-binding protein AFABP ALBP ALBP/Ap2 aP2 Epididymis secretory protein Li 104 FABP FABP4 FABP4_HUMAN Fatty acid binding protein 4 adipocyte Fatty acid binding protein 4 Fatty acid binding protein adipocyte Fatty acid-binding protein 4 Fatty acid-binding protein HEL S 104 Lbpl Myelin P2 protein homolog P15 P2 adipocyte protein Protein 422 |
Images
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Fig1:
Western blot analysis of FABP4 on different lysates with Rabbit anti-FABP4 antibody (ET1703-98) at 1/5,000 dilution and competitor's antibody at 1/5,000 dilution. Lane 1: Mouse white adipose tissue lysate Lane 2: Mouse brown adipose tissue lysate Lane 3: Mouse kidney tissue lysate (negative) Lane 4: Rat white adipose tissue lysate Lane 5: Rat brown adipose tissue lysate Lane 6: Rat kidney tissue lysate (negative) Lysates/proteins at 10 µg/Lane. Predicted band size: 15 kDa Observed band size: 15 kDa Exposure time: 3 minutes 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-98) at 1/5,000 dilution and competitor's antibody at 1/5,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of HAEC cells labeling FABP4 with Rabbit anti-FABP4 antibody (ET1703-98) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-FABP4 antibody (ET1703-98) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.n. |
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Fig3:
Immunofluorescence analysis of frozen mouse adipose tissue labeling FABP4 with Rabbit anti-FABP4 antibody (ET1703-98). The tissues were blocked in 3% BSA for 30 minutes at room temperature, washed with PBS, and then probed with the primary antibody (ET1703-98, green) at 1/100 dilution overnight at 4℃, washed with PBS. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue). Image acquisition was performed with KFBIO KF-FL-400 Scanner. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.