FABP4 Recombinant Rabbit Monoclonal Antibody [JM10-99]
cat.: ET1703-98
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-Fr |
| Clonality: | Monoclonal |
| Clone number: | JM10-99 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 15 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human FABP4 aa 60-100. |
| Positive control: | Mouse white adipose tissue lysate, mouse brown adipose tissue lysate, rat white adipose tissue lysate, rat brown adipose tissue lysate, HAEC, MCF-7, A549, PMVEC. |
| Subcellular location: | Nucleus. Cytoplasm. |
| Recommended Dilutions:
WB IF-Cell IHC-Fr |
1:5,000 1:100 1;100 |
| Uniprot #: | SwissProt: P15090 Human | P04117 Mouse | P70623 Rat |
| Alternative names: | 3T3-L1 lipid-binding protein 422/aP2 A-FABP adipocyte Adipocyte lipid binding protein Adipocyte lipid-binding protein Adipocyte protein AP2 Adipocyte-type fatty acid-binding protein AFABP ALBP ALBP/Ap2 aP2 Epididymis secretory protein Li 104 FABP FABP4 FABP4_HUMAN Fatty acid binding protein 4 adipocyte Fatty acid binding protein 4 Fatty acid binding protein adipocyte Fatty acid-binding protein 4 Fatty acid-binding protein HEL S 104 Lbpl Myelin P2 protein homolog P15 P2 adipocyte protein Protein 422 |
Images
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Fig1:
Western blot analysis of FABP4 on different lysates with Rabbit anti-FABP4 antibody (ET1703-98) at 1/5,000 dilution and competitor's antibody at 1/5,000 dilution. Lane 1: Mouse white adipose tissue lysate Lane 2: Mouse brown adipose tissue lysate Lane 3: Mouse kidney tissue lysate (negative) Lane 4: Rat white adipose tissue lysate Lane 5: Rat brown adipose tissue lysate Lane 6: Rat kidney tissue lysate (negative) Lysates/proteins at 10 µg/Lane. Predicted band size: 15 kDa Observed band size: 15 kDa Exposure time: 3 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-98) at 1/5,000 dilution and competitor's antibody at 1/5,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of HAEC cells labeling FABP4 with Rabbit anti-FABP4 antibody (ET1703-98) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-FABP4 antibody (ET1703-98) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Immunofluorescence analysis of frozen mouse adipose tissue labeling FABP4 with Rabbit anti-FABP4 antibody (ET1703-98). The tissues were blocked in 3% BSA for 30 minutes at room temperature, washed with PBS, and then probed with the primary antibody (ET1703-98, green) at 1/100 dilution overnight at 4℃, washed with PBS. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue). Image acquisition was performed with KFBIO KF-FL-400 Scanner. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.