Mre11 Recombinant Rabbit Monoclonal Antibody [JM11-18]
cat.: ET1703-99
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JM11-18 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 81 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Mre11 aa 465-498 / 708. |
| Positive control: | HeLa cell lysate, 293T cell lysate, K-562 cell lysate, RAW264.7 cell lysate, C6 cell lysate, Mouse testis tissue lysate, Rat testis tissue lysate, human breast cancer tissue, mouse testis tissue, rat testis tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IHC-P |
1:1,000-1:5,000 1:1,000 |
| Uniprot #: | SwissProt: P49959 Human | Q61216 Mouse | Q9JIM0 Rat |
| Alternative names: | AT like disease Ataxia telangiectasia disorder like ATLD DNA recombination and repair protein Double strand break repair protein MRE11A Double-strand break repair protein MRE11A endo/exonuclease Mre11 HNGS1 meiotic recombination (S. cerevisiae) 11 homolog A Meiotic recombination 11 homolog 1 meiotic recombination 11 homolog A (S. cerevisiae) Meiotic recombination 11 homolog A MmMRE11A Mre 11 MRE 11a MRE 11b MRE11 homolog 1 MRE11 homolog A MRE11 meiotic recombination 11 homolog A (S. cerevisiae) MRE11 meiotic recombination 11 homolog A MRE11_HUMAN MRE11A MRE11b OTTHUMP00000236830 OTTHUMP00000236831 OTTHUMP00000236832 OTTHUMP00000236833 |
Images
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Fig1:
Western blot analysis of Mre11 on different lysates with Rabbit anti-Mre11 antibody (ET1703-99) at 1/5,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: 293T cell lysate (20 µg/Lane) Lane 3: K-562 cell lysate (20 µg/Lane) Lane 4: RAW264.7 cell lysate (20 µg/Lane) Lane 5: C6 cell lysate (20 µg/Lane) Lane 6: Mouse testis tissue lysate (40 µg/Lane) Lane 7: Rat testis tissue lysate (40 µg/Lane) Predicted band size: 81 kDa Observed band size: 81 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-99) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-Mre11 antibody (ET1703-99) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-99) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-Mre11 antibody (ET1703-99) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-99) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-Mre11 antibody (ET1703-99) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-99) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.