XRCC1 Recombinant Rabbit Monoclonal Antibody [JA11-47]
cat.: ET1704-01
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P
Clonality: Monoclonal
Clone number: JA11-47
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 70 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human XRCC1 aa 17-53 / 633.
Positive control: Hela cell lysate, mouse testis tissue, human breast infiltrating duct carcinoma tissue, human colon carcinoma tissue.
Subcellular location: Nucleus.
Recommended Dilutions:
  WB
  IHC-P

1:500-1:2,000
1:50-1:200
Uniprot #: SwissProt: P18887 Human | Q60596 Mouse | Q9ESZ0 Rat
Alternative names: DNA repair protein XRCC1 RCC X ray repair complementing defective repair in chinese hamster X ray Repair Complementing Defective Repair in Chinese Hamster Cells X ray repair complementing defective repair in chinese hamster cells 1 X ray repair cross complementing 1 X ray repair cross complementing protein 1 X ray repair, complementing defective, repair in Chinese hamster X ray repair cross-complementing protein 1 XRCC 1 Xrcc1 XRCC1_HUMAN
Images
ET1704-01_1.jpg Fig1: Western blot analysis of XRCC1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1704-01, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Hela cell lysate
Line 2: 293T cell lysate
Line 3: Jarkat cell lysate
ET1704-01_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-XRCC1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-01, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1704-01_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human breast infiltrating duct carcinoma tissue using anti-XRCC1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-01, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1704-01_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-XRCC1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-01, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.