XRCC1 Recombinant Rabbit Monoclonal Antibody [JA11-47]
cat.: ET1704-01
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JA11-47 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 69 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human XRCC1 aa 17-53 / 633. |
| Positive control: | HeLa cell lysate, 293T cell lysate, Jurkat cell lysate, K-562 cell lysate, Mouse colon tissue lysate, Rat colon tissue lysate, Rat testis tissue lysate, mouse testis tissue, rat testis tissue. |
| Subcellular location: | Nucleus, Chromosome. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:2,000 1:1,000 |
| Uniprot #: | SwissProt: P18887 Human | Q60596 Mouse | Q9ESZ0 Rat |
| Alternative names: | DNA repair protein XRCC1 RCC X ray repair complementing defective repair in chinese hamster X ray Repair Complementing Defective Repair in Chinese Hamster Cells X ray repair complementing defective repair in chinese hamster cells 1 X ray repair cross complementing 1 X ray repair cross complementing protein 1 X ray repair, complementing defective, repair in Chinese hamster X ray repair cross-complementing protein 1 XRCC 1 Xrcc1 XRCC1_HUMAN |
Images
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Fig1:
Western blot analysis of XRCC1 on different lysates with Rabbit anti-XRCC1 antibody (ET1704-01) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: 293T cell lysate Lane 3: Jurkat cell lysate Lane 4: K-562 cell lysate Lane 5: Mouse colon tissue lysate Lane 6: Rat colon tissue lysate Lane 7: Rat testis tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 69 kDa Observed band size: 85 kDa Exposure time: 40 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-01) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-XRCC1 antibody (ET1704-01) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-01) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-XRCC1 antibody (ET1704-01) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-01) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.