Calcineurin A Recombinant Rabbit Monoclonal Antibody [JA64-11]
cat.: ET1704-02
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC |
| Clonality: | Monoclonal |
| Clone number: | JA64-11 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 59 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Calcineurin A aa 472-521 / 521. |
| Positive control: | MCF7 cell lysate, mouse brain tissue lysate, rat brain tissue lysate, Hela cell lysate, 293 cell lysate, A431 cell lysate, mouse cerebellum tissue lysate, Hela. |
| Subcellular location: | Cell membrane, Cytoplasm. |
| Recommended Dilutions:
WB FC |
1:500-1:1,000 1:50-1:100 |
| Uniprot #: | SwissProt: Q08209 Human | P63328 Mouse | P63329 Rat |
| Alternative names: | Alpha isoform formerly PPP2B Calcineurin A alpha Calcineurin A1 CalcineurinA Calmodulin dependent calcineurin A subunit alpha isoform Calmodulin-dependent calcineurin A subunit alpha isoform CALN CALNA 1 CALNA CALNA1 CAM PRP catalytic subunit CAM-PRP catalytic subunit CCN 1 CCN1 CNA 1 CNA alpha CNA CNA1 PP2BA_HUMAN PPP2B Ppp3ca Protein phosphatase 2B catalytic subunit Protein phosphatase 3 (formerly 2B) catalytic subunit alpha isoform Protein phosphatase 3 catalytic subunit alpha isoform PPP3CA Protein phosphatase 3 catalytic subunit alpha isozyme Serine/threonine protein phosphatase 2B catalytic subunit alpha isoform Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform |
Images
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Fig1:
Western blot analysis of Calcineurin A on different lysates with Rabbit anti-Calcineurin A antibody (ET1704-02) at 1/1,000 dilution. Lane 1: MCF7 cell lysate (20 µg/Lane) Lane 2: Mouse brain tissue lysate (40 µg/Lane) Lane 3: Rat brain tissue lysate (40 µg/Lane) Predicted band size: 59 kDa Observed band size: 59 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-02) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of Calcineurin A on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1704-02, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: 293 cell lysate Lane 3: A431 cell lysate |
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Fig3:
Western blot analysis of Calcineurin A on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1704-02, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse brain tissue lysate Lane 2: Mouse cerebellum tissue lysate Lane 3: Rat brain tissue lysate |
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Fig4: Flow cytometric analysis of Calcineurin A was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1704-02, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.