Cathepsin B Recombinant Rabbit Monoclonal Antibody [JA11-02]
cat.: ET1704-03
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, IF-Cell, IF-Tissue, IHC-P
Clonality: Monoclonal
Clone number: JA11-02
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: 38 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human Cathepsin B aa 7-176 / 339.
Positive control: SW480 cell lysates, human liver carcinoma tissue, human liver tissue, human pancreas tissue, mouse liver tissue, mouse pancreas tissue, mouse brain tissue.
Subcellular location: Lysosome. Melanosome.
Recommended Dilutions:
  WB
  IF-Cell
  IF-Tissue
  IHC-P
1:500-1:1,000
1:50-1:200
1:50-1:200
1:50-1:200
Uniprot #: SwissProt: P07858 Human
Alternative names: Amyloid precursor protein secretase APP secretase APPS CATB_HUMAN Cathepsin B heavy chain Cathepsin B1 CathepsinB CPSB CTSB cysteine protease OTTHUMP00000116009 OTTHUMP00000229510 OTTHUMP00000229511 OTTHUMP00000229512 OTTHUMP00000229514 OTTHUMP00000229515 OTTHUMP00000229516 Preprocathepsin B
Images
ET1704-03_1.jpg Fig1: Western blot analysis of Cathepsin B on different lysates with Rabbit anti-Cathepsin B antibody (ET1704-03) at 1/1,000 dilution.

Lane 1: HepG2 cell lysate
Lane 2: SW480 cell lysate
Lane 3: Daudi cell lysate (negative)

Lysates/proteins at 20 µg/Lane.

Predicted band size: 38 kDa
Observed band size: 43 kDa

Exposure time: 1 minutes 59 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-03) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET1704-03_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-Cathepsin B antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-03, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1704-03_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Cathepsin B antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-03, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1704-03_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Cathepsin B antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-03, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1704-03_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Cathepsin B antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-03, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1704-03_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-Cathepsin B antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-03, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1704-03_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Cathepsin B antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-03, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.