FGF21 Recombinant Rabbit Monoclonal Antibody [JA10-97]
cat.: ET1704-04
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | JA10-97 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 22 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human FGF21 aa 30-79 / 209. |
| Positive control: | Human liver tissue lysates, mouse spleen tissue lysate, mouse thymus tissue lysate, rat spleen tissue lysate, rat testis tissue lysate, mouse liver tissue, rat liver tissue. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB IHC-P IF-Tissue |
1:1,000-1:5,000 1:1,000 1:200 |
| Uniprot #: | SwissProt: Q9NSA1 Human | Q9JJN1 Mouse Entrez Gene: 170580 Rat |
| Alternative names: | FGF 21 FGF-21 Fgf21 FGF21_HUMAN FGFL Fibroblast growth factor 21 PRO10196 UNQ3115 |
Images
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Fig1:
Western blot analysis of FGF21 on human liver tissue lysates with Rabbit anti-FGF21 antibody (ET1704-04) at 1/2,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 22 kDa Observed band size: 22 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-04) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of FGF21 on different lysates with Rabbit anti-FGF21 antibody (ET1704-04) at 1/2,000 dilution. Lane 1: Mouse spleen tissue lysate Lane 2: Mouse thymus tissue lysate Lane 3: Rat spleen tissue lysate Lane 4: Rat testis tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 22 kDa Observed band size: 22 kDa Exposure time: Lane 1: 20 seconds; Lane 2-4: 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-04) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-FGF21 antibody (ET1704-04) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-04) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-FGF21 antibody (ET1704-04) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-04) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.