ET1704-06

MUC2 Recombinant Rabbit Monoclonal Antibody [JA10-92]

cat.: ET1704-06

Product Type:	Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity:	Human, Mouse, Rat
Applications:	WB, IF-Cell, IF-Tissue, IHC-P
Clonality:	Monoclonal
Clone number:	JA10-92

Form:	Liquid
Storage condition:	Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer:	1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration:	1ug/ul
Purification:	Protein A affinity purified.
Molecular weight:	Predicted band size: 551 kDa
Isotype:	IgG
Immunogen:	Synthetic peptide within Human MUC2 aa 5100-5149 / 5179.
Positive control:	SH-SY5Y cell lysate, SK-Br-3 cell lysate, Hela, HepG2, SW480, human small intestine tissue, mouse small intestine tissue, rat small intestine tissue.
Subcellular location:	Secreted.
Recommended Dilutions: WB IF-Cell IF-Tissue IHC-P	1:1,000 1:100-1:500 1:100-1:500 1:1,000
Uniprot #:	SwissProt: Q02817 Human \| Q80Z19 Mouse \| Q62635 Rat
Alternative names:	Intestinal mucin 2 Intestinal mucin-2 MLP MUC 2 MUC-2 Muc2 MUC2_HUMAN Mucin 2 Mucin 2 intestinal Mucin 2 intestinal/tracheal Mucin 2 oligomeric mucus/gel forming Mucin 2 precursor Mucin 2 tracheal Mucin like protein Mucin-2 Mucin2 SMUC

Images

	Fig1: Western blot analysis of MUC2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1704-06, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SH-SY5Y cell lysate Lane 2: SK-Br-3 cell lysate
	Fig2: Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-MUC2 antibody (ET1704-06) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1704-06) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig3: Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue with Rabbit anti-MUC2 antibody (ET1704-06) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1704-06) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig4: Immunohistochemical analysis of paraffin-embedded rat small intestine tissue with Rabbit anti-MUC2 antibody (ET1704-06) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1704-06) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig5: Application: IF-Tissue Species: Mouse Site: small intestine Sample: Paraffin-embedded section Antibody concentration: 1/100
	Fig6: Immunofluorescence staining of paraffin-embedded human colon tissue using anti-MUC2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with ET1704-06 at 1/100 dilution for 10 hours at 4℃ and detected using Alexa Fluor® 488 conjugate-Goat anti-Rabbit IgG (H+L) Secondary Antibody at a dilution of 1:500 for 1 hour at room temperature.
	Fig7: ICC staining of MUC2 in SW480 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1704-06, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.