MINA53 Recombinant Rabbit Monoclonal Antibody [JA11-63]
cat.: ET1704-07
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IP |
| Clonality: | Monoclonal |
| Clone number: | JA11-63 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 53 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human MINA53 aa 416-465 / 465. |
| Positive control: | HDLM-2 cell lysate, HUT 102 cell lysate, KG-1 cell lysate, A431 cell lysate, A549 cell lysate, Mouse testis tissue lysate, Mouse placenta (E16.5) tissue lysate. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IP |
1:2,000 1-2μg/sample |
| Uniprot #: | SwissProt: Q8IUF8 Human | Q8CD15 Mouse |
| Alternative names: | 60S ribosomal protein L27a histidine hydroxylase Bifunctional lysine specific demethylase and histidyl hydroxylase MINA FLJ14393 Histone lysine demethylase MINA MDIG MINA MINA_HUMAN Mineral dust-induced gene protein Myc induced nuclear antigen 53 kDa MYC induced nuclear antigen MYC-induced nuclear antigen NO52 Nucleolar protein 52 Ribosomal oxygenase MINA ROX |
Images
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Fig1:
Western blot analysis of MINA53 on different lysates with Rabbit anti-MINA53 antibody (ET1704-07) at 1/2,000 dilution. Lane 1: HDLM-2 cell lysate Lane 2: HUT 102 cell lysate Lane 3: KG-1 cell lysate Lane 4: A431 cell lysate Lane 5: A549 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 1 minute 50 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-07) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of MINA53 on different lysates with Rabbit anti-MINA53 antibody (ET1704-07) at 1/2,000 dilution. Lane 1: Mouse testis tissue lysate Lane 2: Mouse placenta (E16.5) tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 13 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-07) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
MINA53 was immunoprecipitated from 0.2 mg A549 cell lysate with ET1704-07 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using ET1704-07 at 1/2,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: A549 cell lysate (input) Lane 2: ET1704-07 IP in A549 cell lysate Lane 3: Rabbit IgG instead of ET1704-07 in A549 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 13 seconds; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.