Vitamin D Receptor Recombinant Rabbit Monoclonal Antibody [JA11-16]
cat.: ET1704-09
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, FC
Clonality: Monoclonal
Clone number: JA11-16
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 48 kDa
Isotype: IgG
Immunogen: Synthetic peptide with Human Vitamin D Receptor aa 1-50 / 427.
Positive control: HL-60 cell lysate, T-47D cell lysate, NIH/3T3 cell lysate, L-929 cell lysate, Hela WT cell lysate, rat large intestine tissue, human skin tissue, mouse colon tissue, mouse skin tissue, Hela, rat skin tissue.
Subcellular location: Nucleus.
Recommended Dilutions:
  WB
  IHC-P
  FC
1:5,000
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: P11473 Human | P48281 Mouse | P13053 Rat
Alternative names: 1 25 dihydroxyvitamin D3 receptor 1 1,25 dihydroxyvitamin D3 receptor 1,25-@dihydroxyvitamin D3 receptor 25-dihydroxyvitamin D3 receptor Member 1 NR1I1 Nuclear receptor subfamily 1 group I member 1 PPP1R163 Protein phosphatase 1, regulatory subunit 163 VDR VDR_HUMAN Vitamin D (1,25- dihydroxyvitamin D3) receptor Vitamin D hormone receptor Vitamin D nuclear receptor variant 1 Vitamin D receptor Vitamin D3 receptor
Images
ET1704-09_1.jpg Fig1: Western blot analysis of Vitamin D Receptor on different lysates with Rabbit anti-Vitamin D Receptor antibody (ET1704-09) at 1/5,000 dilution.

Lane 1: HL-60 cell lysate (15 µg/Lane)
Lane 2: T-47D cell lysate (15 µg/Lane)
Lane 3: NIH/3T3 cell lysate (15 µg/Lane)
Lane 4: L-929 cell lysate (15 µg/Lane)
Lane 5: Rat kidney tissue lysate (20 µg/Lane)

Predicted band size: 48 kDa
Observed band size: 48 kDa

Exposure time: 30 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-09) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET1704-09_2.jpg Fig2: All lanes: Western blot analysis of Vitamin D Receptor with anti-Vitamin D Receptor antibody[JA11-16] (ET1704-09) at 1:500 dilution.
Lane 1: Wild-type Hela whole cell lysate (10 µg).
Lane 2/3: Vitamin D Receptor knockout Hela whole cell lysate (10 µg).

ET1704-09 was shown to specifically react with Vitamin D Receptor in wild-type Hela cells. No bands were observed when Vitamin D Receptor knockout sample were tested. Wild-type and Vitamin D Receptor knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1704-09, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
ET1704-09_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded rat large intestine tissue using anti-Vitamin D Receptor antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-09, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1704-09_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-Vitamin D Receptor antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-09, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1704-09_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-Vitamin D Receptor antibody (ET1704-09) at 1/800 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-09) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1704-09_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-Vitamin D Receptor antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-09, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1704-09_7.jpg Fig7: Flow cytometric analysis of Vitamin D Receptor was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1704-09, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
ET1704-09_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded rat skin tissue with Rabbit anti-Vitamin D Receptor antibody (ET1704-09) at 1/800 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-09) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.