Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human |
Applications: | WB |
Clonality: | Monoclonal |
Clone number: | JA93-11 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | 140 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide within Human TLR7 aa 1000-1049 / 1049. |
Positive control: | Daudi cell lysates. |
Subcellular location: | Endoplasmic reticulum membrane. |
Recommended Dilutions:
WB |
1:500-1:1,000 |
Uniprot #: | SwissProt: Q9NYK1 Human |
Alternative names: | PRO285 TLR 7 Tlr7 TLR7_HUMAN Toll like receptor 7 Toll-like receptor 7 UNQ248 |
Fig1: Western blot analysis of TLR7 on Daudi cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1704-11, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |