Rhodopsin Recombinant Rabbit Monoclonal Antibody [JA10-91]
cat.: ET1704-12
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JA10-91 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 39 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Rhodopsin aa 299-348 / 348. |
| Positive control: | Rat retina tissue. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:1,000 1:50-1:200 |
| Uniprot #: | SwissProt: P08100 Human | P51489 Rat |
| Alternative names: | CSNBAD1 MGC138309 MGC138311 OPN 2 OPN2 opsd OPSD_HUMAN opsin 2 Opsin 2 rod pigment Opsin-2 Opsin2 Retinitis Pigmentosa 4 Retinitis pigmentosa 4 autosomal dominant RHO Rhodopsin RP 4 RP4 |
Images
|
Fig1: Immunohistochemical analysis of paraffin-embedded rat retina tissue using anti-Rhodopsin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-12, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.