Rhodopsin Recombinant Rabbit Monoclonal Antibody [JA10-91]
cat.: ET1704-12
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IHC-Fr |
| Clonality: | Monoclonal |
| Clone number: | JA10-91 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 39 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Rhodopsin aa 299-348 / 348. |
| Positive control: | Rat retina tissue. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB IHC-P IHC-Fr |
1:1,000 1:1,000 1:200-1:500 |
| Uniprot #: | SwissProt: P08100 Human | P51489 Rat |
| Alternative names: | CSNBAD1 MGC138309 MGC138311 OPN 2 OPN2 opsd OPSD_HUMAN opsin 2 Opsin 2 rod pigment Opsin-2 Opsin2 Retinitis Pigmentosa 4 Retinitis pigmentosa 4 autosomal dominant RHO Rhodopsin RP 4 RP4 |
Images
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Fig1:
Western blot analysis of Rhodopsin on different lysates with Rabbit anti-Rhodopsin antibody (ET1704-12) at 1/1,000 dilution. Lane 1: Rat eye tissue lysate Lane 2: Rat liver tissue lysate Lysates/proteins at 30 µg/Lane. Exposure time: 1 minute; ECL: K1801 The multiple bands correspond to dimers and multimers of Rhodopsin, consistent with the literature (PMID: 25270370). Negative expression of Rhodopsin protein in liver tissue is consistent with the predicted expression pattern. Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ET1704-12, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature |
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Fig2:
Application: Immunohistochemistry (IHC-P) Species: Mouse Tissue: Retina Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: ET1704-12, 1/1,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
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Fig3:
Application: Immunohistochemistry (IHC-P) Species: Rat Tissue: Retina Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: ET1704-12, 1/1,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
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Fig4:
Application: Immunofluorescence (IHC-Fr) Species: Mouse Tissue: Retina Sample: Frozen section Antigen retrieval: Not required Wash buffer: 1× TBST Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: ET1704-12, 1/200, overnight at 4℃. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature. |
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Fig5:
Application: Immunofluorescence (IHC-Fr) Species: Rat Tissue: Retina Sample: Frozen section Antigen retrieval: Not required Wash buffer: 1× TBST Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: ET1704-12, 1/500, overnight at 4℃. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.