Anti-MHC Class II antibody [JA10-94]
cat.: ET1704-13
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, IP, ICC/IF, IHC-P
Clonality: Monoclonal
Clone number: JA10-94
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 29 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human HLA-DPB1 aa 20-60.
Positive control: Human tonsil tissue, human spleen tissue.
Subcellular location: Cell membrane. Endoplasmic reticulum membrane.
Recommended Dilutions:
  WB
  ICC/IF
  IHC-P
  IP

1:500-1:1,000
1:50-1:100
1:50-1:200
Use at an assay dependent concentration.
Uniprot #: SwissProt: P04440 Human
Alternative names: D6S221E antibody DMA antibody DMB antibody DP beta 1 antibody DP beta 1 chain antibody DP(W4) beta chain antibody DPB 1 antibody DPB1 antibody DPB1_HUMAN antibody DRB antibody H2Ea antibody HLA class II histocompatibility antigen antibody HLA class II histocompatibility antigen DM beta chain antibody HLA class II histocompatibility antigen, DP beta 1 chain antibody HLA class II histocompatibility antigen, DP(W4) beta chain antibody HLA DMB antibody HLA DP1A antibody HLA DPB1 antibody HLA SB alpha chain antibody HLA-A antibody HLA-A histocompatibility type antibody HLA-DP antibody HLA-DP histocompatibility type, beta-1 subunit antibody HLA-DP1B antibody HLA-DPB antibody HLA-DPB1 antibody HLADM antibody HLADP1B antibody HLASB antibody HLASB histocompatibility type antibody Human MHC class II HLA SB alpha antibody LA class II histocompatibility antigen DP alpha 1 chain antibody Major histocompatibility complex class II antibody Major histocompatibility complex class II DP alpha 1 antibody Major histocompatibility complex class II DP beta 1 antibody Major histocompatibility complex, class I, A antibody MHC class II antigen DMB antibody MHC class II antigen DPB1 antibody MHC class II DP3 alpha antibody MHC class II DPA1 antibody MHC class II HLA-DP-beta-1 antibody MHC DPB1 antibody MHC HLA DPB1 antibody PLT1 antibody Primed lymphocyte test 1 antibody RING6 antibody RING7 antibody
Images
ET1704-13_1.jpg Fig1: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-MHC Class II antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-13, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1704-13_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-MHC Class II antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-13, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.