MHC Class II Recombinant Rabbit Monoclonal Antibody [JA10-94]
cat.: ET1704-13
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, IP, IF-Cell, IF-Tissue, IHC-P, mIHC
Clonality: Monoclonal
Clone number: JA10-94
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 29 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human HLA-DPB1 aa 11-60 / 258.
Positive control: Daudi cell lysates, human tonsil tissue, human spleen tissue.
Subcellular location: Cell membrane. Endoplasmic reticulum membrane.
Recommended Dilutions:
  WB
  IF-Cell
  IF-Tissue
  IHC-P
  IP
  mIHC
1:500-1:1,000
1:50-1:100
1:50-1:100
1:50-1:200
Use at an assay dependent concentration.
1:2,000
Uniprot #: SwissProt: P04440 Human
Alternative names: D6S221E DMA DMB DP beta 1 DP beta 1 chain DP(W4) beta chain DPB 1 DPB1 DPB1_HUMAN DRB H2Ea HLA class II histocompatibility antigen HLA class II histocompatibility antigen DM beta chain HLA class II histocompatibility antigen, DP beta 1 chain HLA class II histocompatibility antigen, DP(W4) beta chain HLA DMB HLA DP1A HLA DPB1 HLA SB alpha chain HLA-A HLA-A histocompatibility type HLA-DP HLA-DP histocompatibility type, beta-1 subunit HLA-DP1B HLA-DPB HLA-DPB1 HLADM HLADP1B HLASB HLASB histocompatibility type Human MHC class II HLA SB alpha LA class II histocompatibility antigen DP alpha 1 chain Major histocompatibility complex class II Major histocompatibility complex class II DP alpha 1 Major histocompatibility complex class II DP beta 1 Major histocompatibility complex, class I, A MHC class II antigen DMB MHC class II antigen DPB1 MHC class II DP3 alpha MHC class II DPA1 MHC class II HLA-DP-beta-1 MHC DPB......
Images
ET1704-13_1.jpg Fig1: Western blot analysis of MHC Class II on Daudi cell lysates with Rabbit anti-MHC Class II antibody (ET1704-13) at 1/500 dilution.

Lysates/proteins at 10 µg/Lane.

Predicted band size: 29 kDa
Observed band size: 29 kDa

Exposure time: 1 minute;

12% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-13) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
ET1704-13_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-MHC Class II antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-13, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1704-13_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-MHC Class II antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-13, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1704-13_4.jpg Fig4: Fluorescence multiplex immunohistochemical analysis of Human tonsil (Formalin/PFA-fixed paraffin-embedded sections). Panel A: the merged image of anti-BCL6 (HA601083, Red), anti-HLA-DR (ET1704-13, Green), anti-Tryptase (ET1610-64, White), anti-CD20 (HA721138, Magenta) and anti-CD45 (ET7111-03, Yellow) on tonsil. HRP Conjugated UltraPolymer Goat Polyclonal Antibody HA1119/HA1120 was used as a secondary antibody. The immunostaining was performed with the Sequential Immuno-staining Kit (IRISKit™MH010101, www.luminiris.cn). The section was incubated in five rounds of staining: in the order of HA601083 (1/200 dilution), ET1704-13 (1/2,000 dilution), ET1610-64 (1/5,000 dilution), HA721138 (1/2,000 dilution) and ET7111-03 (1/500 dilution) for 20 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.