MHC Class II Recombinant Rabbit Monoclonal Antibody [JA10-94]
cat.: ET1704-13
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IP, IF-Cell, IF-Tissue, IHC-P, mIHC |
| Clonality: | Monoclonal |
| Clone number: | JA10-94 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 29 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human HLA-DPB1 aa 11-60 / 258. |
| Positive control: | Daudi cell lysates, human tonsil tissue. |
| Subcellular location: | Cell membrane. Endoplasmic reticulum membrane. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue IHC-P IP mIHC |
1:500-1:1,000 1:50-1:100 1:50-1:100 1:1,000 Use at an assay dependent concentration. 1:2,000 |
| Uniprot #: | SwissProt: P04440 Human |
| Alternative names: | D6S221E DMA DMB DP beta 1 DP beta 1 chain DP(W4) beta chain DPB 1 DPB1 DPB1_HUMAN DRB H2Ea HLA class II histocompatibility antigen HLA class II histocompatibility antigen DM beta chain HLA class II histocompatibility antigen, DP beta 1 chain HLA class II histocompatibility antigen, DP(W4) beta chain HLA DMB HLA DP1A HLA DPB1 HLA SB alpha chain HLA-A HLA-A histocompatibility type HLA-DP HLA-DP histocompatibility type, beta-1 subunit HLA-DP1B HLA-DPB HLA-DPB1 HLADM HLADP1B HLASB HLASB histocompatibility type Human MHC class II HLA SB alpha LA class II histocompatibility antigen DP alpha 1 chain Major histocompatibility complex class II Major histocompatibility complex class II DP alpha 1 Major histocompatibility complex class II DP beta 1 Major histocompatibility complex, class I, A MHC class II antigen DMB MHC class II antigen DPB1 MHC class II DP3 alpha MHC class II DPA1 MHC class II HLA-DP-beta-1 MHC DPB...... |
Images
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Fig1:
Western blot analysis of MHC Class II on Daudi cell lysates with Rabbit anti-MHC Class II antibody (ET1704-13) at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 29 kDa Observed band size: 29 kDa Exposure time: 1 minute; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-13) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-MHC Class II antibody (ET1704-13) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-13) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Fluorescence multiplex immunohistochemical analysis of human tonsil (Formalin/PFA-fixed paraffin-embedded sections). Panel A: the merged image of anti-HLA-DBP1 (ET1704-13, Green) and anti-Tryptase (ET1610-64, Red) on tonsil. HRP Conjugated UltraPolymer Goat Polyclonal Antibody HA1119/HA1120 was used as a secondary antibody. The immunostaining was performed with the Sequential Immuno-staining Kit (IRISKit™MH010101, www.luminiris.cn). The section was incubated in two rounds of staining: in the order of ET1704-13 (1/2,000 dilution) and ET1610-64 (1/5,000 dilution) for 20 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Zeiss Observer 7 Inverted Fluorescence Microscope. |
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Fig4: Fluorescence multiplex immunohistochemical analysis of Human tonsil (Formalin/PFA-fixed paraffin-embedded sections). Panel A: the merged image of anti-BCL6 (HA601083, Red), anti-HLA-DPB1 (ET1704-13, Green), anti-Tryptase (ET1610-64, White), anti-CD20 (HA721138, Magenta) and anti-CD45 (ET7111-03, Yellow) on tonsil. HRP Conjugated UltraPolymer Goat Polyclonal Antibody HA1119/HA1120 was used as a secondary antibody. The immunostaining was performed with the Sequential Immuno-staining Kit (IRISKit™MH010101, www.luminiris.cn). The section was incubated in five rounds of staining: in the order of HA601083 (1/200 dilution), ET1704-13 (1/2,000 dilution), ET1610-64 (1/5,000 dilution), HA721138 (1/2,000 dilution) and ET7111-03 (1/500 dilution) for 20 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.