NFAT1 Recombinant Rabbit Monoclonal Antibody [JA11-08]
cat.: ET1704-14
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Tissue, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JA11-08 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 100 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human NFAT1 aa 21-70 / 925. |
| Positive control: | Jurkat cell lysate, Ramos cell lysate, Raji cell lysate, Daudi cell lysate, human spleen tissue, human NK-T-cell lymphoma tissue, human colon cancer tissue. |
| Subcellular location: | Cytoplasm. Nucleus. |
| Recommended Dilutions:
WB IF-Tissue IHC-P |
1:1,000 1:50-1:200 1:1,000 |
| Uniprot #: | SwissProt: Q13469 Human |
| Alternative names: | AI607462 cytoplasmic 2 KIAA0611 NF ATc2 NF ATp NF-ATc2 NF-ATp NFAC2_HUMAN NFAT 1 NFAT pre existing subunit NFAT pre-existing subunit NFAT transcription complex, preexisting component NFAT1 NFAT1-D NFATc2 NFATp Nuclear factor of activated T cells cytoplasmic 2 Nuclear factor of activated T cells cytoplasmic calcineurin dependent 2 Nuclear factor of activated T cells pre-existing component Nuclear factor of activated T cells, preexisting component Nuclear factor of activated T-cells Preexisting nuclear factor of activated T cells 2 T cell transcription factor NFAT 1 T-cell transcription factor NFAT1 |
Images
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Fig1:
Western blot analysis of NFAT1 on different lysates with Rabbit anti-NFAT1 antibody (ET1704-14) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: Ramos cell lysate Lane 3: Raji cell lysate Lane 4: Daudi cell lysate Lane 5: HeLa cell lysate (negative) Lysates/proteins at 30 µg/Lane. Predicted band size: 100 kDa Observed band size: 120/140 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-14) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-NFAT1 antibody (ET1704-14) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-14) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human NK-T-cell lymphoma tissue with Rabbit anti-NFAT1 antibody (ET1704-14) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-14) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-NFAT1 antibody (ET1704-14) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-14) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.