PKA 2 beta (regulatory subunit) Recombinant Rabbit Monoclonal Antibody [JA11-69]
cat.: ET1704-15
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IP, IF-Cell, IF-Tissue, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JA11-69 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 46 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human PKA 2 beta aa 369-418 / 418. |
| Positive control: | Hela, N2A, SH-SY5Y, mouse brain tissue. |
| Subcellular location: | Cytoplasm. Cell membrane. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue IHC-P FC |
1:500-1:1,000 1:50-1:200 1:50-1:200 1:1,000 1:50-1:100 |
| Uniprot #: | SwissProt: P31323 Human | P31324 Mouse | P12369 Rat |
| Alternative names: | AI451071 AW061005 cAMP dependent protein kinase type II beta regulatory chain cAMP dependent protein kinase type II beta regulatory subunit cAMP-dependent protein kinase type II-beta regulatory subunit H RG363E19.2 KAP3_HUMAN MGC116401 Pkarb2 PRKAR 2 PRKAR2 PRKAR2B Protein kinase cAMP dependent regulatory type II beta RATDNA RII beta RII(beta) RIIbeta WUGSC:H RG363E19.2 |
Images
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Fig1: ICC staining of PKA 2 beta (regulatory subunit) in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1704-15, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig2: ICC staining of PKA 2 beta (regulatory subunit) in N2A cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1704-15, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig3: ICC staining of PKA 2 beta (regulatory subunit) in SH-SY5Y cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1704-15, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig4:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-PKA 2 beta (regulatory subunit) antibody (ET1704-15) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-15) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5: Flow cytometric analysis of PKA 2 beta (regulatory subunit) was done on N2A cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1704-15, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.