PRKAR2B (regulatory subunit) Recombinant Rabbit Monoclonal Antibody [JA11-69]
cat.: ET1704-15
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IP, IF-Cell, IF-Tissue, IHC-P, FC
Clonality: Monoclonal
Clone number: JA11-69
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 46 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human PRKAR2B aa 369-418 / 418.
Positive control: Rat brain tissue lysates, mouse brain tissue, rat brain tissue, Hela, N2A, SH-SY5Y.
Subcellular location: Cytoplasm. Cell membrane.
Recommended Dilutions:
  WB
  IF-Cell
  IF-Tissue
  IHC-P
  FC
1:2,000
1:50-1:200
1:50-1:200
1:200-1:1,000
1:50-1:100
Uniprot #: SwissProt: P31323 Human | P31324 Mouse | P12369 Rat
Alternative names: AI451071 AW061005 cAMP dependent protein kinase type II beta regulatory chain cAMP dependent protein kinase type II beta regulatory subunit cAMP-dependent protein kinase type II-beta regulatory subunit H RG363E19.2 KAP3_HUMAN MGC116401 Pkarb2 PRKAR 2 PRKAR2 PRKAR2B Protein kinase cAMP dependent regulatory type II beta RATDNA RII beta RII(beta) RIIbeta WUGSC:H RG363E19.2
Images
ET1704-15_1.jpg Fig1: Western blot analysis of PRKAR2B (regulatory subunit) on rat brain tissue lysates with Rabbit anti-PRKAR2B (regulatory subunit) antibody (ET1704-15) at 1/2,000 dilution.

Lysates/proteins at 40 µg/Lane.

Predicted band size: 46 kDa
Observed band size: 50 kDa

Exposure time: 1 minute; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-15) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET1704-15_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-PRKAR2B (regulatory subunit) antibody (ET1704-15) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-15) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1704-15_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-PRKAR2B (regulatory subunit) antibody (ET1704-15) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-15) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1704-15_4.jpg Fig4: ICC staining of PRKAR2B (regulatory subunit) in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1704-15, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1704-15_5.jpg Fig5: ICC staining of PRKAR2B (regulatory subunit) in N2A cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1704-15, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1704-15_6.jpg Fig6: ICC staining of PRKAR2B (regulatory subunit) in SH-SY5Y cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1704-15, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1704-15_7.jpg Fig7: Flow cytometric analysis of PRKAR2B (regulatory subunit) was done on N2A cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1704-15, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
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