ADFP/PLIN2 Recombinant Rabbit Monoclonal Antibody [JA31-81]
cat.: ET1704-17
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IF-Tissue, FC, IHC-P
Clonality: Monoclonal
Clone number: JA31-81
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 48 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human ADFP aa 18-67 / 437.
Positive control: HepG2 cell lysate, JAR cell lysate, Human liver tissue lysate, HeLa, human liver tissue, human liver carcinoma tissue, human stomach tissue.
Subcellular location: Membrane.
Recommended Dilutions:
  WB
  IF-Cell
  IF-Tissue
  FC
  IHC-P
1:1,000
1:50-1:200
1:50-1:200
1:1,000
1:100
Uniprot #: SwissProt: Q99541 Human | P43883 Mouse
Entrez Gene: 298199 Rat
Alternative names: ADFP Adipophilin Adipose differentiation related protein Adipose differentiation-related protein ADRP MGC10598 Perilipin-2 PLIN2 PLIN2_HUMAN
Images
ET1704-17_1.jpg Fig1: Western blot analysis of ADFP/PLIN2 on different lysates with Rabbit anti-ADFP/PLIN2 antibody (ET1704-17) at 1/1,000 dilution.

Lane 1: HepG2 cell lysate
Lane 2: JAR cell lysate
Lane 3: Human liver tissue lysate

Lysates/proteins at 40 µg/Lane.

Predicted band size: 48 kDa
Observed band size: 45 kDa

Exposure time: 1 minute 2 seconds; ECL: K1802;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-17) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET1704-17_2.jpg Fig2: Immunocytochemistry analysis of HeLa cells labeling ADFP/PLIN2 with Rabbit anti-ADFP/PLIN2 antibody (ET1704-17) at 1/50 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-ADFP/PLIN2 antibody (ET1704-17) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
ET1704-17_3.jpg Fig3: Flow cytometric analysis of HeLa cells labeling ADFP/PLIN2.

Cells were fixed and permeabilized. Then stained with the primary antibody (ET1704-17, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
ET1704-17_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-ADFP/PLIN2 antibody (ET1704-17) at 1/100 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-17) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1704-17_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue with Rabbit anti-ADFP/PLIN2 antibody (ET1704-17) at 1/100 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-17) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1704-17_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human stomach tissue with Rabbit anti-ADFP/PLIN2 antibody (ET1704-17) at 1/100 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-17) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.