Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse |
Applications: | WB, ICC/IF, IHC-P |
Clonality: | Monoclonal |
Clone number: | JA52-11 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1 mg/mL. |
Purification: | Protein A affinity purified. |
Molecular weight: | 60 kDa |
Isotype: | IgG |
Immunogen: | Recombinant protein within human Tyrosinase aa 1-300. |
Positive control: | A431, B16F1, MCF-7, human liver tissue, human stomach carcinoma tissue. |
Subcellular location: | Melanosome membrane, Melanosome. |
Recommended Dilutions:
WB ICC/IF IHC-P |
1:500-1:1,000 1:100-1:500 1:50-1:200 |
Uniprot #: | SwissProt: P14679 Human | P11344 Mouse |
Alternative names: | ATN antibody CMM8 antibody LB24 AB antibody LB24-AB antibody Monophenol monooxygenase antibody OCA1 antibody OCA1A antibody OCAIA antibody Oculocutaneous albinism IA antibody SHEP3 antibody SK29 AB antibody SK29-AB antibody Tumor rejection antigen AB antibody TYR antibody TYRO_HUMAN antibody tyrosinase (oculocutaneous albinism IA) antibody Tyrosinase antibody |
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Fig1: ICC staining of Tyrosinase in A431 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1704-18, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig2: ICC staining of Tyrosinase in B16F1 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1704-18, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig3: ICC staining of Tyrosinase in MCF-7 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1704-18, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig4: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Tyrosinase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-18, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human stomach carcinoma tissue using anti-Tyrosinase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-18, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |