PRMT1 Recombinant Rabbit Monoclonal Antibody [JA11-17]
cat.: ET1704-19
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IF-Tissue, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JA11-17 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 42 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human PRMT1 aa 12-61 / 371. |
| Positive control: | Human white adipose tissue, mouse colon tissue, D3, NIH/3T3, Hela, HepG2, SW480. |
| Subcellular location: | Nucleus. Cytoplasm. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue IHC-P |
1:500-1:1,000 1:100-1:500 1:100-1:500 1:200 |
| Uniprot #: | SwissProt: Q99873 Human | Q9JIF0 Mouse | Q63009 Rat |
| Alternative names: | ANM 1 ANM1 ANM1_HUMAN HCP 1 HCP1 Heterogeneous nuclear ribonucleoprotein methyltransferase 1 like 2 Heterogeneous nuclear ribonucleoproteins methyltransferase like 2 Heterogeneous nuclear ribonucleoproteins methyltransferase like2 Histone-arginine N-methyltransferase PRMT1 HMT 2 HMT1 (hnRNP methyltransferase S. cerevisiae) like 2 HMT1 hnRNP methyltransferase HMT1 hnRNP methyltransferase like 2 (S. cerevisiae) HMT1 hnRNP methyltransferase like 2 HMT2 HRMT1 L2 HRMT1L 2 HRMT1L2 Human mRNA for suppressor for yeast mutant Human mRNA for suppressor for yeast mutant complete cds Interferon receptor 1 bound protein 4 Interferon receptor 1 bound protein4 Interferon receptor 1-bound protein 4 Interferon receptor 1bound protein 4 IR1 B4 IR1B 4 IR1B4 Mrmt 1 Mrmt1 PRMT 1 PRMT1 Protein arginine methyltransferase 1 Protein arginine N methyltransferase 1 Protein arginine N methyltransferase1 Protein arginine N-methyltransferase 1 R1...... |
Images
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Fig1:
Immunohistochemical analysis of paraffin-embedded human white adipose tissue with Rabbit anti-PRMT1 antibody (ET1704-19) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-19) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-PRMT1 antibody (ET1704-19) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-19) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: ICC staining of PRMT1 in D3 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1704-19, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig4: ICC staining of PRMT1 in NIH/3T3 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1704-19, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig5: ICC staining of PRMT1 in Hela cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1704-19, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig6: ICC staining of PRMT1 in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1704-19, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig7: ICC staining of PRMT1 in SW480 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1704-19, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.