PRMT1 Recombinant Rabbit Monoclonal Antibody [JA11-17]
cat.: ET1704-19
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IF-Tissue, IHC-P
Clonality: Monoclonal
Clone number: JA11-17
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: 42 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human PRMT1 aa 12-61 / 371.
Positive control: D3, NIH/3T3, Hela, HepG2, SW480, human breast carcinoma tissue, mouse liver tissue, mouse colon tissue.
Subcellular location: Nucleus. Cytoplasm.
Recommended Dilutions:
  WB
  IF-Cell
  IF-Tissue
  IHC-P
1:500-1:1,000
1:100-1:500
1:100-1:500
1:50-1:200
Uniprot #: SwissProt: Q99873 Human | Q9JIF0 Mouse | Q63009 Rat
Alternative names: ANM 1 ANM1 ANM1_HUMAN HCP 1 HCP1 Heterogeneous nuclear ribonucleoprotein methyltransferase 1 like 2 Heterogeneous nuclear ribonucleoproteins methyltransferase like 2 Heterogeneous nuclear ribonucleoproteins methyltransferase like2 Histone-arginine N-methyltransferase PRMT1 HMT 2 HMT1 (hnRNP methyltransferase S. cerevisiae) like 2 HMT1 hnRNP methyltransferase HMT1 hnRNP methyltransferase like 2 (S. cerevisiae) HMT1 hnRNP methyltransferase like 2 HMT2 HRMT1 L2 HRMT1L 2 HRMT1L2 Human mRNA for suppressor for yeast mutant Human mRNA for suppressor for yeast mutant complete cds Interferon receptor 1 bound protein 4 Interferon receptor 1 bound protein4 Interferon receptor 1-bound protein 4 Interferon receptor 1bound protein 4 IR1 B4 IR1B 4 IR1B4 Mrmt 1 Mrmt1 PRMT 1 PRMT1 Protein arginine methyltransferase 1 Protein arginine N methyltransferase 1 Protein arginine N methyltransferase1 Protein arginine N-methyltransferase 1 R1......
Images
ET1704-19_1.jpg Fig1: ICC staining of PRMT1 in D3 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1704-19, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1704-19_2.jpg Fig2: ICC staining of PRMT1 in NIH/3T3 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1704-19, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1704-19_3.jpg Fig3: ICC staining of PRMT1 in Hela cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1704-19, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1704-19_4.jpg Fig4: ICC staining of PRMT1 in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1704-19, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1704-19_5.jpg Fig5: ICC staining of PRMT1 in SW480 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1704-19, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1704-19_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-PRMT1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-19, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1704-19_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-PRMT1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-19, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1704-19_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-PRMT1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-19, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.