Phosphotyrosine Recombinant Rabbit Monoclonal Antibody [JA10-49]
cat.: ET1704-20
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Species independent |
| Applications: | WB, IF-Cell, IF-Tissue, IP, FC |
| Clonality: | Monoclonal |
| Clone number: | JA10-49 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | CXXXX-pY-XXXXNH2. |
| Positive control: | A431 cells lysates treated with Sodium metavanadate. |
| Recommended Dilutions:
WB FC IF-Cell IF-Tissue |
1:50-1:200 1:10-1:50 1:50 1:50 |
Images
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Fig1: Western blot analysis of Phosphotyrosine on A431 cells lysates treated (Line 1) and untreated with Sodium metavanadate using anti- Phosphotyrosine antibody at 1/100 dilution. |
|
Fig2: ICC staining Phosphotyrosine in A431 cells (green) nontreated (Left) or treated with Sodium metavanadate (Right). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
|
Fig3: Flow cytometric analysis of A431 cells with Phosphotyrosine antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.