gamma Sarcoglycan Recombinant Rabbit Monoclonal Antibody [JA11-57]
cat.: ET1704-24
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse
Applications: WB, IP, IHC-P
Clonality: Monoclonal
Clone number: JA11-57
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 32 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human gamma Sarcoglycan aa 42-291 / 291.
Positive control: Human skeletal muscle tissue lysates, human striated muscle tissue.
Subcellular location: Cell membrane. Cytoplasm.
Recommended Dilutions:
  WB
  IHC-P
  IP

1:500-1:2,000
1:50-1:200
Use at an assay dependent concentration.
Uniprot #: SwissProt: Q13326 Human
Alternative names: 35 kDa dystrophin associated glycoprotein 35 kDa dystrophin-associated glycoprotein 35DAG 35kD dystrophin associated glycoprotein 35kDa dystrophin-associated glycoprotein A4 DAGA4 DMDA DMDA1 Gamma SG Gamma-sarcoglycan Gamma-SG LGMD2C MAM MGC130048 Sarcoglycan gamma SCARMD2 SCG3 SGCG SGCG_HUMAN TYPE
Images
ET1704-24_1.jpg Fig1: Western blot analysis of gamma Sarcoglycan on human skeletal muscle tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1704-24, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
ET1704-24_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human striated muscle tissue with Rabbit anti-gamma Sarcoglycan antibody (ET1704-24) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-24) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.