gamma Sarcoglycan Recombinant Rabbit Monoclonal Antibody [JA11-57]
cat.: ET1704-24
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IP, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JA11-57 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 32 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human gamma Sarcoglycan aa 42-291 / 291. |
| Positive control: | Human skeletal muscle tissue lysates, human striated muscle tissue. |
| Subcellular location: | Cell membrane. Cytoplasm. |
| Recommended Dilutions:
WB IHC-P IP |
1:500-1:2,000 1:50-1:200 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: Q13326 Human |
| Alternative names: | 35 kDa dystrophin associated glycoprotein 35 kDa dystrophin-associated glycoprotein 35DAG 35kD dystrophin associated glycoprotein 35kDa dystrophin-associated glycoprotein A4 DAGA4 DMDA DMDA1 Gamma SG Gamma-sarcoglycan Gamma-SG LGMD2C MAM MGC130048 Sarcoglycan gamma SCARMD2 SCG3 SGCG SGCG_HUMAN TYPE |
Images
|
Fig1:
Immunohistochemical analysis of paraffin-embedded human striated muscle tissue with Rabbit anti-gamma Sarcoglycan antibody (ET1704-24) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-24) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig2:
Western blot analysis of gamma Sarcoglycan on different lysates with Rabbit anti-gamma Sarcoglycan antibody (ET1704-24) at 1/1,000 dilution. Lane 1: MCF-7 (Human breast cancer cell) cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 25 seconds; ECL: K1802 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ET1704-24, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 32 kDa Observed band size: 32 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.