alpha sarcoglycan Recombinant Rabbit Monoclonal Antibody [JA51-81]
cat.: ET1704-25
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IP, IHC-P
Clonality: Monoclonal
Clone number: JA51-81
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 43 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human alpha sarcoglycan aa 338-387 / 387.
Positive control: Mouse heart tissue lysate, human skeletal muscle tissue lysate, mouse heart tissue, mouse skeletal muscle tissue, human striated muscle tissue, rat skeletal muscle tissue.
Subcellular location: Sarcolemma, cytoskeleton.
Recommended Dilutions:
  WB
  IHC-P
  IP

1:500-1:2,000
1:50-1:1,000
Use at an assay dependent concentration.
Uniprot #: SwissProt: Q16586 Human | P82350 Mouse | Q5SWB2 Rat
Alternative names: 50 DAG 50 kDa dystrophin associated glycoprotein 50 kDa dystrophin-associated glycoprotein 50DAG 50kD DAG 59kDa A2 adhalin ADL Alpha SG Alpha-sarcoglycan Alpha-SG Asg DAG2 DMDA2 Dystroglycan 2 Dystroglycan-2 LGMD2D sarcoglycan, alpha (dystrophin-associated glycoprotein) SCARMD1 Sgca SGCA_HUMAN
Images
ET1704-25_1.jpg Fig1: Western blot analysis of alpha sarcoglycan on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1704-25, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: mouse heart tissue lysate
Lane 2: human skeletal muscle tissue lysate
ET1704-25_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-alpha sarcoglycan antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-25, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1704-25_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-alpha sarcoglycan antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-25, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1704-25_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human striated muscle tissue with Rabbit anti-alpha sarcoglycan antibody (ET1704-25) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-25) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1704-25_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue with Rabbit anti-alpha sarcoglycan antibody (ET1704-25) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-25) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.