Lin28 Recombinant Rabbit Monoclonal Antibody [JA10-63]
cat.: ET1704-26
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IP, FC |
| Clonality: | Monoclonal |
| Clone number: | JA10-63 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 23 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Lin28 aa 10-59 / 209. |
| Positive control: | NCCIT cell lysates, Hela, K562 cell lysates, rat testis tissue, human testis tissue, mouse placenta tissue. |
| Subcellular location: | Cytoplasm. Nucleus. |
| Recommended Dilutions:
WB IHC-P IP FC |
1:500-1:1,000 1:400 1:10-1:50 1:50-1:100 |
| Uniprot #: | SwissProt: Q9H9Z2 Human | Q8K3Y3 Mouse | D3ZZA6 Rat |
| Alternative names: | AL024421 CSDD1 CSDD2 FLJ12457 Lin 28 Lin 28 homolog (C. elegans) Lin 28 homolog A (C. elegans) Lin 28 homolog A Lin 28 homolog Lin-28A Lin28 Lin28, C. elegans, homolog of, A LIN28A LN28A_HUMAN Protein lin-28 homolog A Protein lin-28 homolog B RNA binding protein lin 28 Tex17 ZCCHC1 Zinc finger CCHC domain containing 1 Zinc finger CCHC domain containing protein 1 Zinc finger CCHC domain-containing protein 1 |
Images
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Fig1:
Western blot analysis of Lin28 on NCCIT cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1704-26, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Predicted band size: 23 kDa Observed band size: 25 kDa |
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Fig2: Flow cytometric analysis of Lin28 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1704-26, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig3:
Western blot analysis of Lin28 on K562 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1704-26, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Predicted band size: 23 kDa Observed band size: 25 kDa |
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Fig4:
Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-Lin28 antibody (ET1704-26) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-26) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-Lin28 antibody (ET1704-26) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-26) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded mouse placenta tissue with Rabbit anti-Lin28 antibody (ET1704-26) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-26) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.