IP10 Recombinant Rabbit Monoclonal Antibody [JA10-82]
cat.: ET1704-27
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, IF-Cell, IF-Tissue, IHC-P
Clonality: Monoclonal
Clone number: JA10-82
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 11 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human IP10 aa 49-98 / 98.
Positive control: THP-1 treated with 100ng/mL IFN-γ for 24 hours then 300ng/mL Brefeldin A for 20 hours cell lysate, THP-1 treated with 200ng/mL IFN-γ and 50ng/mL LPS for 24 hours cell lysate, HepG2, SH-SY5Y, human skin tissue, human tonsil tissue.
Subcellular location: Secreted.
Recommended Dilutions:
  WB
  IF-Cell
  IF-Tissue
  IHC-P
1:1,000
1:50-1:100
1:50-1:100
1:50-1:1,000
Uniprot #: SwissProt: P02778 Human
Alternative names: Interferon gamma induced factor MOB1, mouse, homolog of Interferon gamma induced protein 10 10 kDa interferon gamma induced protein 10 kDa interferon gamma-induced protein C X C motif chemokine 10 C7 Chemokine (C X C motif) ligand 10 Chemokine CXC motif ligand 10 Crg 2 CRG2 CXCL10 CXCL10(1-73) CXL10_HUMAN Gamma IP10 Gamma-IP10 gIP 10 GIP10 IFI10 INP 10 INP10 Interferon activated gene 10 Interferon activated gene 10 Interferon gamma induced cell line Interferon inducible cytokine IP 10 Interferon inducible cytokine IP10 IP 10 IP-10 Mob 1 MOB1 Protein 10 from interferon (gamma) induced cell line SCYB10 Small inducible cytokine B10 Small inducible cytokine B10 precursor Small inducible cytokine subfamily B (Cys X Cys) member 10 Small inducible cytokine subfamily B CXC member 10 Small inducible cytokine subfamily B, member 10 Small-inducible cytokine B10
Images
ET1704-27_1.jpg Fig1: Western blot analysis of IP10 on different lysates with Rabbit anti-IP10 antibody (ET1704-27) at 1/1,000 dilution.

Lane 1: THP-1 cell lysate
Lane 2: THP-1 treated with 100ng/mL IFN-γ for 24 hours then 300ng/mL Brefeldin A for 20 hours cell lysate
Lane 3: THP-1 cell lysate
Lane 4: THP-1 treated with 200ng/mL IFN-γ and 50ng/mL LPS for 24 hours cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 11 kDa
Observed band size: 11 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-27) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET1704-27_2.jpg Fig2: ICC staining of IP10 in HepG2 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1704-27, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1704-27_3.jpg Fig3: ICC staining of IP10 in SH-SY5Y cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1704-27, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1704-27_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-IP10 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-27, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1704-27_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-IP10 antibody (ET1704-27) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-27) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1704-27_6.jpg Fig6: Immunocytochemistry analysis of THP-1 cells treated with 200ng/mL IFN-γ then treated with 50ng/mL LPS for 24 hours labeling IP10 with Rabbit anti-IP10 antibody (ET1704-27) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-IP10 antibody (ET1704-27) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.