SFRP1 Recombinant Rabbit Monoclonal Antibody [JA11-68]
cat.: ET1704-29
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JA11-68 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 35 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human SFRP1 aa 31-80 / 314. |
| Positive control: | SH-SY5Y cell lysate, SK-MEL-28 cell lysate, NIH/3T3 cell lysate, Mouse kidney tissue lysate, human testis tissue. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:1,000 |
| Uniprot #: | SwissProt: Q8N474 Human | Q8C4U3 Mouse |
| Alternative names: | Frizzled related protein 1 FRP 1 FRP FRP-1 FRP1 FrzA SARP 2 SARP-2 SARP2 Secreted apoptosis related protein 2 Secreted apoptosis-related protein 2 Secreted frizzled related protein 1 Secreted frizzled related protein Secreted frizzled-related protein 1 SFRP 1 sFRP-1 SFRP1 SFRP1_HUMAN |
Images
|
Fig1:
Western blot analysis of SFRP1 on different lysates with Rabbit anti-SFRP1 antibody (ET1704-29) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate (20 µg/Lane) Lane 2: SK-MEL-28 cell lysate (20 µg/Lane) Lane 3: NIH/3T3 cell lysate (20 µg/Lane) Lane 4: Mouse kidney tissue lysate (40 µg/Lane) Predicted band size: 35 kDa Observed band size: 35 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-29) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-SFRP1 antibody (ET1704-29) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-29) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.