Integrin alpha 4 Recombinant Rabbit Monoclonal Antibody [JA09-36]
cat.: ET1704-31
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JA09-36 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 115 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Integrin alpha 4 aa 983-1032 / 1032. |
| Positive control: | Jurkat cell lysate, mouse spleen tissue lysate, rat spleen tissue lysate, RAW264.7 cell lysate, human tonsil tissue, human spleen tissue, Jurkat. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB IHC-P FC |
1:1,000 1:50-1:200 1:10-1:50 |
| Uniprot #: | SwissProt: P13612 Human | Q00651 Mouse Entrez Gene: 311144 Rat |
| Alternative names: | 269C wild type Antigen CD49D, alpha 4 subunit of VLA 4 receptor CD49 antigen like family member D CD49 antigen-like family member D CD49d IA4 Integrin alpha 4 Integrin alpha 4 subunit Integrin alpha IV Integrin alpha-4 Integrin alpha-IV Integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA 4 receptor) ITA4_HUMAN ITGA4 MGC90518 OTTHUMP00000205320 Very late activation protein 4 receptor, alpha 4 subunit VLA 4 subunit alpha VLA-4 subunit alpha VLA4 |
Images
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Fig1:
Western blot analysis of Integrin alpha 4 on different lysates with Rabbit anti-Integrin alpha 4 antibody (ET1704-31) at 1/1,000 dilution. Lane 1: Jurkat cell lysate (20 µg/Lane) Lane 2: Mouse spleen tissue lysate (40 µg/Lane) Lane 3: Rat spleen tissue lysate (40 µg/Lane) Predicted band size: 115 kDa Observed band size: 70/140/150 kDa Exposure time: Lane 1: 25 seconds; Lane 2-3: 5 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-31) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of Integrin alpha 4 on different lysates with Rabbit anti-Integrin alpha 4 antibody (ET1704-31) at 1/1,000 dilution. Lane 1: RAW264.7 cell lysate Lane 2: RAW264.7 cell lysate treated with deglycosylation Lysates/proteins at 20 µg/Lane. Predicted band size: 115 kDa Observed band size: 70/140/150 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-31) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Integrin alpha 4 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-31, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Integrin alpha 4 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-31, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5: Flow cytometric analysis of Integrin alpha 4 was done on Jurkat cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1704-31, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.