Integrin alpha 4 Recombinant Rabbit Monoclonal Antibody [JA09-36]
cat.: ET1704-31
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC |
| Clonality: | Monoclonal |
| Clone number: | JA09-36 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 115 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Integrin alpha 4 aa 983-1032 / 1032. |
| Positive control: | Jurkat cell lysate, Mouse spleen tissue lysate, Rat spleen tissue lysate, RAW264.7 cell lysate, Jurkat. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB FC |
1:1,000 1:10-1:50 |
| Uniprot #: | SwissProt: P13612 Human | Q00651 Mouse Entrez Gene: 311144 Rat |
| Alternative names: | 269C wild type Antigen CD49D, alpha 4 subunit of VLA 4 receptor CD49 antigen like family member D CD49 antigen-like family member D CD49d IA4 Integrin alpha 4 Integrin alpha 4 subunit Integrin alpha IV Integrin alpha-4 Integrin alpha-IV Integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA 4 receptor) ITA4_HUMAN ITGA4 MGC90518 OTTHUMP00000205320 Very late activation protein 4 receptor, alpha 4 subunit VLA 4 subunit alpha VLA-4 subunit alpha VLA4 |
Images
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Fig1:
Western blot analysis of Integrin alpha 4 on different lysates with Rabbit anti-Integrin alpha 4 antibody (ET1704-31) at 1/1,000 dilution. Lane 1: Jurkat cell lysate (20 µg/Lane) Lane 2: Mouse spleen tissue lysate (40 µg/Lane) Lane 3: Rat spleen tissue lysate (40 µg/Lane) Predicted band size: 115 kDa Observed band size: 70/140/150 kDa Exposure time: Lane 1: 25 seconds; Lane 2-3: 5 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-31) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of Integrin alpha 4 on different lysates with Rabbit anti-Integrin alpha 4 antibody (ET1704-31) at 1/1,000 dilution. Lane 1: RAW264.7 cell lysate Lane 2: RAW264.7 cell lysate treated with deglycosylation Lysates/proteins at 20 µg/Lane. Predicted band size: 115 kDa Observed band size: 70/140/150 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-31) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3: Flow cytometric analysis of Integrin alpha 4 was done on Jurkat cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1704-31, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.