GATA4 Recombinant Rabbit Monoclonal Antibody [JA11-41]
cat.: ET1704-32
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IF-Tissue, FC
Clonality: Monoclonal
Clone number: JA11-41
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: 54 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human GATA 4 aa 5-54.
Positive control: Hela cell lysate, 293 cell lysate, rat liver tissue lysate, PC-3 cell lysate, PC-3M, Hela.
Subcellular location: Nucleus.
Recommended Dilutions:
  WB
  IF-Cell
  IF-Tissue
  FC

1:500-1:1,000
1:50-1:200
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: P43694 Human | Q08369 Mouse | P46152 Rat
Alternative names: ASD2 GATA 4 GATA binding protein 4 GATA-binding factor 4 gata4 GATA4_HUMAN MGC126629 Transcription factor GATA 4 Transcription factor GATA-4 Transcription factor GATA4 VSD1
Images
ET1704-32_1.jpg Fig1: Western blot analysis of GATA4 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1704-32, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 2: Hela cell lysate
Lane 2: 293 cell lysate
Lane 1: Rat liver tissue lysate
Lane 2: PC-3 cell lysate
ET1704-32_2.jpg Fig2: ICC staining of GATA4 in PC-3M cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1704-32, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1704-32_3.jpg Fig3: Flow cytometric analysis of GATA4 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1704-32, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.