GATA4 Recombinant Rabbit Monoclonal Antibody [JA11-41]
cat.: ET1704-32
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | JA11-41 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 45 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human GATA4 aa 5-54. |
| Positive control: | HeLa cell lysate, HEK-293 cell lysate, PC-3 cell lysate, Mouse ovary tissue lysate, Mouse testis tissue lysate, Rat ovary tissue lysate, Rat testis tissue lysate, HEK-293, Hela. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IF-Cell FC |
1:1,000 1:250 1:50-1:100 |
| Uniprot #: | SwissProt: P43694 Human | Q08369 Mouse | P46152 Rat |
| Alternative names: | ASD2 GATA 4 GATA binding protein 4 GATA-binding factor 4 gata4 GATA4_HUMAN MGC126629 Transcription factor GATA 4 Transcription factor GATA-4 Transcription factor GATA4 VSD1 |
Images
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Fig1:
Western blot analysis of GATA4 on different lysates with Rabbit anti-GATA4 antibody (ET1704-32) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: HEK-293 cell lysate (20 µg/Lane) Lane 3: PC-3 cell lysate (20 µg/Lane) Lane 4: Mouse ovary tissue lysate (40 µg/Lane) Lane 5: Mouse testis tissue lysate (40 µg/Lane) Lane 6: Rat ovary tissue lysate (40 µg/Lane) Lane 7: Rat testis tissue lysate (40 µg/Lane) Predicted band size: 45 kDa Observed band size: 55 kDa Exposure time: 15 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-32) at 1/1,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of HEK-293 cells labeling GATA4 with Rabbit anti-GATA4 antibody (ET1704-32) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-GATA4 antibody (ET1704-32) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3: Flow cytometric analysis of GATA4 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1704-32, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.