Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
---|---|
Species reactivity: | Human, Mouse, Rat |
Applications: | WB, IHC-P, FC |
Clonality: | Monoclonal |
Clone number: | JA30-03 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | 6 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide within Human Noxa aa 5-54 / 54. |
Positive control: | Human spleen tissue, human liver tissue, Jurkat, mouse lung tissue, rat lung tissue. |
Subcellular location: | Mitochondrion. |
Recommended Dilutions:
WB IHC-P FC |
1:500 1:50-1:8,000 1:50 |
Uniprot #: | SwissProt: Q13794 Human | Q9JM54 Mouse | Q5U777 Rat |
Alternative names: | Adult T cell leukemia derived PMA responsive APR APR_HUMAN ATL-derived Immediate early response protein APR Immediate-early-response protein APR NOXA Phorbol 12 myristate 13 acetate induced protein 1 Phorbol-12-myristate-13-acetate-induced protein 1 PMA induced protein 1 PMA-induced protein 1 PMA-responsive gene Pmaip1 Protein Noxa |
Fig1: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Noxa antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-35, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. | |
Fig2: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Noxa antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-35, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. | |
Fig3: Flow cytometric analysis of Noxa was done on Jurkat cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1704-35, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). | |
Fig4:
Immunohistochemical analysis of paraffin-embedded mouse lung tissue with Rabbit anti-Noxa antibody (ET1704-35) at 1/8,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-35) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Fig5:
Immunohistochemical analysis of paraffin-embedded rat lung tissue with Rabbit anti-Noxa antibody (ET1704-35) at 1/8,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-35) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |