Noxa Recombinant Rabbit Monoclonal Antibody [JA30-03]
cat.: ET1704-35
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JA30-03 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 6 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Noxa aa 5-54 / 54. |
| Positive control: | Human spleen tissue, human liver tissue, Jurkat, mouse lung tissue, rat lung tissue. |
| Subcellular location: | Mitochondrion. |
| Recommended Dilutions:
WB IHC-P |
1:500 1:8,000 |
| Uniprot #: | SwissProt: Q13794 Human | Q9JM54 Mouse | Q5U777 Rat |
| Alternative names: | Adult T cell leukemia derived PMA responsive APR APR_HUMAN ATL-derived Immediate early response protein APR Immediate-early-response protein APR NOXA Phorbol 12 myristate 13 acetate induced protein 1 Phorbol-12-myristate-13-acetate-induced protein 1 PMA induced protein 1 PMA-induced protein 1 PMA-responsive gene Pmaip1 Protein Noxa |
Images
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Fig1:
Immunohistochemical analysis of paraffin-embedded mouse lung tissue with Rabbit anti-Noxa antibody (ET1704-35) at 1/8,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-35) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded rat lung tissue with Rabbit anti-Noxa antibody (ET1704-35) at 1/8,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-35) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.