CaMKII delta Recombinant Rabbit Monoclonal Antibody [JA30-07]
cat.: ET1704-37
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Zebrafish |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JA30-07 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 56 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human CaMKII delta aa 22-71 / 499. |
| Positive control: | Human skeletal muscle tissue lysate, rat heart tissue lysate, zebrafish tissue, rat brain tissue, mouse skeletal muscle tissue, mouse heart tissue. |
| Subcellular location: | Sarcolemma. Sarcoplasmic reticulum membrane. |
| Recommended Dilutions:
WB IHC-P |
1:1,000-1:2,000 1:50-1:200 |
| Uniprot #: | SwissProt: Q13557 Human | Q6PHZ2 Mouse | P15791 Rat |
| Alternative names: | Calcium / calmodulin dependent protein kinase 2 delta Calcium / calmodulin dependent protein kinase II delta calcium/calmodulin-dependent protein kinase (CaM kinase) II delta calcium/calmodulin-dependent protein kinase type II delta chain Calcium/calmodulin-dependent protein kinase type II subunit delta CAM kinase 2 delta CAM kinase II delta CaM kinase II delta subunit CaM kinase II subunit delta CaM-kinase II delta chain CAMK 2d CaMK-II delta subunit CaMK-II subunit delta CAMK2D CAMKD CAMKI KCC2D_HUMAN RATCAMKI |
Images
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Fig1:
Western blot analysis of CaMKII delta on different lysates with Rabbit anti-CaMKII delta antibody (ET1704-37) at 1/2,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-CaMKII delta KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 56 kDa Observed band size: 50 kDa Exposure time: 100 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-37) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of CaMKII delta on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1704-37, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: human skeletal muscle tissue lysate Lane 2: rat heart tissue lysate |
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Fig3: Immunohistochemical analysis of paraffin-embedded zebrafish tissue using anti-CaMKII delta antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-37, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-CaMKII delta antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-37, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5: Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-CaMKII delta antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-37, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-CaMKII delta antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-37, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.