GCLC Recombinant Rabbit Monoclonal Antibody [JA08-03]
cat.: ET1704-38
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Rat
Applications: WB, IHC-P, FC
Clonality: Monoclonal
Clone number: JA08-03
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 73 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human GCLC aa 588-637 / 637.
Positive control: A431 cell lysate, A549 cell lysate, Rat kidney tissue lysate, human fetal kidney tissue, human fetal liver tissue, rat kidney tissue, Jurkat.
Subcellular location: Cytoplasm.
Recommended Dilutions:
  WB
  IHC-P
  FC
1:500-1:1,000
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: P48506 Human | P19468 Rat
Alternative names: EC 6.3.2.2 Gamma ECS Gamma glutamylcysteine synthetase Gamma-ECS antibod Gamma-glutamylcysteine synthetase GCL Gclc GCS GCS heavy chain GLCL GLCLC Glutamate cysteine ligase catalytic subunit Glutamate--cysteine ligase catalytic subunit GSH1_HUMAN
Images
ET1704-38_1.jpg Fig1: Western blot analysis of GCLC on different lysates with Rabbit anti-GCLC antibody (ET1704-38) at 1/1,000 dilution.

Lane 1: A431 cell lysate (20 µg/Lane)
Lane 2: A549 cell lysate (20 µg/Lane)
Lane 3: Rat kidney tissue lysate (40 µg/Lane)

Predicted band size: 73 kDa
Observed band size: 73 kDa

Exposure time: 1 minute; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-38) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET1704-38_2.jpg Fig2: Western blot analysis of GCLC on different lysates with Rabbit anti-GCLC antibody (ET1704-38) at 1/5,000 dilution.

Lane 1: A549-si NT cell lysate
Lane 2: A549-si GCLC cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 73 kDa
Observed band size: 73 kDa

Exposure time: 2 minutes; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-38) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET1704-38_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human fetal kidney tissue with Rabbit anti-GCLC antibody (ET1704-38) at 1/50 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-38) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1704-38_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human fetal liver tissue with Rabbit anti-GCLC antibody (ET1704-38) at 1/50 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-38) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1704-38_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-GCLC antibody (ET1704-38) at 1/50 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-38) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1704-38_6.jpg Fig6: Flow cytometric analysis of GCLC was done on Jurkat cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1704-38, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.