Glucose 6 Phosphate Dehydrogenase Recombinant Rabbit Monoclonal Antibody [JA31-40]
cat.: ET1704-39
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JA31-40 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 59 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Glucose 6 Phosphate Dehydrogenase aa 460-509 / 515. |
| Positive control: | A549 cell lysate, Hela, HepG2, MCF-7, human liver tissue, human stomach cancer tissue, mouse testes tissue, mouse skeletal muscle tissue. |
| Subcellular location: | Cytoplasm. Cytoplasmic side of plasma membrane. Membrane. Nucleus. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500-1:2,000 1:50-1:200 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: P11413 Human |
| Alternative names: | G6PD G6PD_HUMAN G6PD1 G6pdx Glucose 6 phosphate 1 dehydrogenase Glucose 6 phosphate dehydrogenase Glucose 6 phosphate dehydrogenase, G6PD Glucose-6-phosphate 1-dehydrogenase MET19 POS10 Zwf1p |
Images
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Fig1:
Western blot analysis of Glucose 6 Phosphate Dehydrogenase on different lysates with Rabbit anti-Glucose 6 Phosphate Dehydrogenase antibody (ET1704-39) at 1/1,000 dilution. Lane 1: HCT 116-si NT cell lysate Lane 2: HCT 116-si G6PD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 59 kDa Observed band size: 55 kDa Exposure time: 1 minute 40 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-39) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2: Western blot analysis of Glucose 6 Phosphate Dehydrogenase on A549 cell lysate using anti-Glucose 6 Phosphate Dehydrogenase antibody at 1/1,000 dilution. |
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Fig3: ICC staining Glucose 6 Phosphate Dehydrogenase in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: Immunohistochemical analysis of paraffin-embedded mouse testes tissue using anti-Glucose 6 Phosphate Dehydrogenase antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Glucose 6 Phosphate Dehydrogenase antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-Glucose 6 Phosphate Dehydrogenase antibody. Counter stained with hematoxylin. |
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Fig7: Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-Glucose 6 Phosphate Dehydrogenase antibody. Counter stained with hematoxylin. |
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Fig8: Flow cytometric analysis of Hela cells with Glucose 6 Phosphate Dehydrogenase antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.