Anti-IKK gamma antibody [JA11-83]
cat.: ET1704-40
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, ICC/IF, IP, FC
Clonality: Monoclonal
Clone number: JA11-83
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 48 kDa
Isotype: IgG
Immunogen: Recombinant protein within human IKK gamma aa 1-400.
Positive control: Hela cell lysates, Hela, human spleen tissue, human colon carcinoma tissue, mouse colon tissue, mouse fallopian tissue, human colon tissue, human placenta tissue, mouse heart tissue.
Subcellular location: Nucleus. Cytoplasm.
Recommended Dilutions:
  WB
  ICC/IF
  IHC-P
  FC
  IP

1:500-1:2,000
1:50-1:200
1:20-1:50
1:50-1:100
Use at an assay dependent concentration.
Uniprot #: SwissProt: Q9Y6K9 Human | O88522 Mouse | Q6TMG5 Rat
Alternative names: IkB kinase associated protein 1 antibody IkB kinase subunit gamma antibody Inhibitor of nuclear factor kappa B kinase subunit gamma antibody AMCBX1 antibody FIP 3 antibody FIP-3 antibody FIP3 antibody Fip3p antibody I kappa B kinase gamma antibody I-kappa-B kinase subunit gamma antibody IkB kinase gamma subunit antibody IkB kinase subunit gamma antibody IkB kinase-associated protein 1 antibody Ikbkg antibody IKK-gamma antibody IKKAP1 antibody IKKG antibody IMD33 antibody Incontinentia pigmenti antibody Inhibitor of kappa light polypeptide gene enhancer in B cells, kinase gamma antibody Inhibitor of kappa light polypeptide gene enhancer in B cells, kinase of, gamma antibody Inhibitor of nuclear factor kappa-B kinase subunit gamma antibody IP antibody IP1 antibody IP2 antibody IPD2 antibody NEMO antibody NEMO_HUMAN antibody NF kappa B essential modifier antibody NF kappa B essential modulator antibody NF-kappa-B essential modifier antibody NF-kappa-B essential modulator antibody ZC2HC9 antibody
Images
ET1704-40_1.jpg Fig1: Western blot analysis of IKK gamma on Hela cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1704-40, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
ET1704-40_2.jpg Fig2: ICC staining of IKK gamma in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1704-40, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1704-40_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-IKK gamma antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-40, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1704-40_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-IKK gamma antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-40, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1704-40_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-IKK gamma antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-40, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1704-40_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse fallopian tissue using anti-IKK gamma antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-40, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1704-40_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-IKK gamma antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-40, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1704-40_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-IKK gamma antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-40, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1704-40_9.jpg Fig9: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-IKK gamma antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-40, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1704-40_10.jpg Fig10: Flow cytometric analysis of IKK gamma was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1704-40, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.