Anti-Profilin 1 antibody [JA30-13]
cat.: ET1704-42
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, ICC, IHC-P, FC
Clonality: Monoclonal
Clone number: JA30-13
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A purified.
Molecular weight: 15 kDa
Isotype: IgG
Immunogen: recombinant protein
Positive control: HepG2, Hela, A549, human kidney tissue, mouse colon tissue, mouse kidney tissue.
Subcellular location: Cytoplasm, cytoskeleton.
Recommended Dilutions:
  WB
  ICC
  IHC-P
  FC

1:500-1:2,000
1:50-1:200
1:50-1:200
1:50-1:100
Uniprot #: P07737(Human) P62962(Mouse) P62963(Rat)
Alternative names: Actin binding protein antibody ALS18 antibody Epididymis tissue protein Li 184a antibody OTTHUMP00000125244 antibody PFN 1 antibody Pfn antibody PFN1 antibody PROF1_HUMAN antibody Profilin I antibody Profilin-1 antibody Profilin1 antibody ProfilinI antibody
Images
ET1704-42_1.jpg Fig1: Western blot analysis of Profilin 1 on Hela cell using anti-Profilin 1 antibody at 1/1,000 dilution.
ET1704-42_2.jpg Fig2: ICC staining Profilin 1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET1704-42_3.jpg Fig3: ICC staining Profilin 1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET1704-42_4.jpg Fig4: ICC staining Profilin 1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET1704-42_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Profilin 1 antibody. Counter stained with hematoxylin.
ET1704-42_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Profilin 1 antibody. Counter stained with hematoxylin.
ET1704-42_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Profilin 1 antibody. Counter stained with hematoxylin.
ET1704-42_8.jpg Fig8: Flow cytometric analysis of Hela cells with Profilin 1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.