Leptin Receptor Recombinant Rabbit Monoclonal Antibody [JA73-01]
cat.: ET1704-44
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IHC-P, FC
Clonality: Monoclonal
Clone number: JA73-01
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 132 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human Leptin Receptor aa 1041-1090 / 1165.
Positive control: Mouse lung tissue lysate, mouse liver tissue lysate, K-562 cell lysates, HeLa cell lysates, rat brain tissue lysates, rat heart tissue, human liver carcinoma tissue, human prostate tissue, human kidney tissue, mouse small intestine tissue, K-562.
Subcellular location: Cell membrane, Basolateral cell membrane.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  FC
1:500-1:2,000
1:50-1:100
1:50-1:1,000
1:1,000
Uniprot #: SwissProt: P48357 Human | P48356 Mouse | Q62959 Rat
Alternative names: CD 295 CD295 CD295 antigen Db Fa HuB219 LEP R LEP-R LEPR LEPR_HUMAN LEPRD Leptin receptor Leptin receptor fatty Leptin receptor gene related protein Leptin receptor precursor Leptin receptor precursor OB R gene related protein OB receptor OB-R OB-RGRP obl Obr
Images
ET1704-44_1.jpg Fig1: Western blot analysis of Leptin Receptor on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1704-44, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Mouse lung tissue lysate
Lane 2: Mouse liver tissue lysate
ET1704-44_2.jpg Fig2: Western blot analysis of Leptin Receptor on different lysates with Rabbit anti-Leptin Receptor antibody (ET1704-44) at 1/1,000 dilution.

Lane 1: K-562 cell lysate (16 µg/Lane)
Lane 2: HeLa cell lysate (20 µg/Lane)
Lane 3: Rat brain tissue lysate (32 µg/Lane)

Predicted band size: 132 kDa
Observed band size: 132 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-44) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
ET1704-44_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-Leptin Receptor antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-44, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1704-44_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-Leptin Receptor antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-44, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1704-44_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-Leptin Receptor antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-44, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1704-44_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Leptin Receptor antibody (ET1704-44) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-44) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1704-44_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-Leptin Receptor antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-44, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1704-44_8.jpg Fig8: Immunocytochemistry analysis of K-562 cells labeling Leptin Receptor with Rabbit anti-Leptin Receptor antibody (ET1704-44) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Leptin Receptor antibody (ET1704-44) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
ET1704-44_9.jpg Fig9: Flow cytometric analysis of K-562 cells labeling Leptin Receptor.

Cells were fixed and permeabilized. Then stained with the primary antibody (ET1704-44, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
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