NFAT2 Recombinant Rabbit Monoclonal Antibody [JA81-03]
cat.: ET1704-45
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JA81-03 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 101 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human NFAT2 aa 894-943 / 943. |
| Positive control: | K-562 cell lysates, human tonsil tissue, K-562. |
| Subcellular location: | Cytoplasm. Nucleus. |
| Recommended Dilutions:
WB IHC-P FC |
1:500-1:1,000 1:50-1:200 1:1,000 |
| Uniprot #: | SwissProt: O95644 Human |
| Alternative names: | cytoplasmic 1 MGC138448 NF ATc NF ATc1 NF-ATc NF-ATc1 NF-ATc1.2 NFAC1_HUMAN NFAT 2 NFAT transcription complex cytosolic component NFATC 1 NFATc NFATc1 Nuclear factor of activated T cells cytoplasmic 1 Nuclear factor of activated T cells cytoplasmic calcineurin dependent 1 Nuclear factor of activated T cells cytosolic component 1 nuclear factor of activated T-cells 'c' Nuclear factor of activated T-cells |
Images
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Fig1: Western blot analysis of NFAT2 on K-562 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1704-45, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-NFAT2 antibody (ET1704-45) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-45) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Flow cytometric analysis of K-562 cells labeling NFAT2. Cells were fixed and permeabilized. Then stained with the primary antibody (ET1704-45, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.