GDNF Recombinant Rabbit Monoclonal Antibody [JA93-10]
cat.: ET1704-46
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | JA93-10 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 24 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human GDNF aa 51-100/211. |
| Positive control: | HepG2 cell lysates, SH-SY5Y. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB IF-Cell |
1:500 1:100 |
| Uniprot #: | SwissProt: P39905 Human | P48540 Mouse | Q07731 Rat |
| Alternative names: | Astrocyte derived trophic factor Astrocyte derived trophic factor 1 Astrocyte-derived trophic factor ATF 1 ATF 2 Atf ATF1 ATF2 gdnf GDNF_HUMAN Glial cell derived neurotrophic factor Glial Cell Line Derived Neurotrophic Factor Glial cell line-derived neurotrophic factor Glial derived neurotrophic factor HFB1 GDNF hGDNF HSCR3 |
Images
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Fig1:
Western blot analysis of GDNF on different lysates with Rabbit anti-GDNF antibody (ET1704-46) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: Neuro-2a cell lysate Lane 3: C6 cell lysate Lane 4: Mouse skeletal muscle tissue lysate Lane 5: Mouse brain tissue lysate Lane 6: Rat brain tissue lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 24 kDa Observed band size: 30 kDa Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-46) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunocytochemistry analysis of C6 cells labeling GDNF with Rabbit anti-GDNF antibody (ET1704-46) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-GDNF antibody (ET1704-46) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Western blot analysis of GDNF on HepG2 cell lysates with Rabbit anti-GDNF antibody (ET1704-46) at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 24 kDa Observed band size: 26 kDa Exposure time: 1 minute; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-46) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.