Annexin A2 Recombinant Rabbit Monoclonal Antibody [JA42-30]
cat.: ET1704-49
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, IF-Cell, IF-Tissue, FC
Clonality: Monoclonal
Clone number: JA42-30
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 39 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human Annexin A2 aa 26-75 / 339.
Positive control: HeLa (Human cervical adenocarcinoma cell) cell lysate, LNCaP (Human prostate cancer cell) cell lysate, MCF7 (Human breast cancer cell) cell lysate, NIH/3T3 (Mouse fibroblast) cell lysate, 4T1 (Mouse breast cancer cell) cell lysate, C6 (Rat glioma cell) cell lysate, PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate, mouse heart tissue lysate, mouse kidney tissue lysate, rat brain tissue lysate, rat heart tissue lysate, rat kidney tissue lysate.
Subcellular location: Basement membrane. Melanosome.
Recommended Dilutions:
  WB
  IHC-P
  IF-Cell
  IF-Tissue
  FC
1:50,000
1:50-1:200
1:100-1:200
1:100-1:200
1:50-1:200
Uniprot #: SwissProt: P07355 Human | P07356 Mouse | Q07936 Rat
Alternative names: Annexin A2 Annexin II Annexin II, heavy chain Annexin-2 ANX 2 ANX2 ANX2L4 ANXA2 ANXA2_HUMAN arylsulfatase B CAL1H Calpactin I heavy chain calpactin I heavy polypeptide (p36) Calpactin I heavy polypeptide Calpactin-1 heavy chain chromobindin 8 Chromobindin-8 Epididymis secretory protein Li 270 HEL S 270 LIP2 Lipocortin II LPC2 LPC2D p36 P36 protein PAP-IV Placental anticoagulant protein IV Protein I
Images
ET1704-49_1.jpg Fig1: Western blot analysis of Annexin A2 on different lysates with Rabbit anti-Annexin A2 antibody (ET1704-49) at 1/50,000 dilution.

Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate
Lane 2: LNCaP (Human prostate cancer cell) cell lysate
Lane 3: MCF7 (Human breast cancer cell) cell lysate
Lane 4: NIH/3T3 (Mouse fibroblast) cell lysate
Lane 5: 4T1 (Mouse breast cancer cell) cell lysate
Lane 6: C6 (Rat glioma cell) cell lysate
Lane 7: PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate

Lysates/proteins at 15 µg/Lane.
Exposure time: 7 seconds; ECL: K1801

Low expression of Annexin A2 protein in LNCaP is consistent with the predicted expression pattern.

Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: ET1704-49, 1/50,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

Predicted band size: 39 kDa
Observed band size: 34 kDa
ET1704-49_2.jpg Fig2: Western blot analysis of Annexin A2 on different lysates with Rabbit anti-Annexin A2 antibody (ET1704-49) at 1/50,000 dilution.

Lane 1: Mouse heart tissue lysate
Lane 2: Mouse kidney tissue lysate
Lane 3: Rat brain tissue lysate
Lane 4: Rat heart tissue lysate
Lane 5: Rat kidney tissue lysate

Lysates/proteins at 15 µg/Lane.
Exposure time: 7 seconds; ECL: K1801

Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: ET1704-49, 1/50,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

Predicted band size: 39 kDa
Observed band size: 34 kDa
ET1704-49_3.jpg Fig3: Western blot analysis of Annexin A2 on different lysates with Rabbit anti-Annexin A2 antibody (ET1704-49) at 1/1,000 dilution.

Lane 1: A549-WT cell lysate
Lane 2: A549-KD Annexin A2 cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 39 kDa
Observed band size: 34 kDa

Exposure time: 12 seconds; ECL: K1801;
4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-49) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET1704-49_4.jpg Fig4: Application: Immunohistochemistry (IHC-P)

Species: Human
Tissue: Kidney
Sample: Paraffin-embedded section

Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.

Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: ET1704-49, 1/1,000, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature.
ET1704-49_5.jpg Fig5: Application: Immunohistochemistry (IHC-P)

Species: Mouse
Tissue: Kidney
Sample: Paraffin-embedded section

Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.

Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: ET1704-49, 1/1,000, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature.
ET1704-49_6.jpg Fig6: Application: Immunohistochemistry (IHC-P)

Species: Rat
Tissue: Kidney
Sample: Paraffin-embedded section

Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.

Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: ET1704-49, 1/1,000, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature.
ET1704-49_7.jpg Fig7: ICC staining of Annexin A2 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1704-49, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1704-49_8.jpg Fig8: Flow cytometric analysis of Annexin A2 was done on K562 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1704-49, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.