Clathrin heavy chain Recombinant Rabbit Monoclonal Antibody [JA53-02]
cat.: ET1704-50
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Monkey |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JA53-02 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 180 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Clathrin heavy chain aa 201-250 / 1,675. |
| Positive control: | HeLa cell lysate, A431 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, HUVEC cell lysate, SH-SY5Y cell lysate, COS-1 cell lysate, human colon cancer tissue. |
| Subcellular location: | Cytoplasmic vesicle membrane. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:200 |
| Uniprot #: | SwissProt: Q00610 Human | Q68FD5 Mouse | P11442 Rat |
| Alternative names: | CHC17 Clathrin heavy chain Clathrin heavy chain 1 Clathrin heavy chain 2 Clathrin heavy chain like 1 Clathrin heavy chain on chromosome 17 Clathrin heavy polypeptide CLH 17 CLH 22 CLH-17 CLH1_HUMAN CLH17 CLH22 CLTC CLTCL CLTCL1 CLTCL2 CLTD Hc KIAA0034 |
Images
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Fig1:
Western blot analysis of Clathrin heavy chain on different lysates with Rabbit anti-Clathrin heavy chain antibody (ET1704-50) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: A431 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: PC-12 cell lysate Lane 5: HUVEC cell lysate Lane 6: SH-SY5Y cell lysate Lane 7: COS-1 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 180kDa Observed band size: 180 kDa Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-50) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-Clathrin heavy chain antibody (ET1704-50) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-50) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.