hnRNP A1 Recombinant Rabbit Monoclonal Antibody [JA39-21]
cat.: ET1704-52
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IHC-P, FC
Clonality: Monoclonal
Clone number: JA39-21
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: 39 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human hnRNP A1 aa 301-350 / 372.
Positive control: HepG2 cell lysate, PC-12 cell lysate, MCF-7, Hela, HepG2, human tonsil tissue, human colon carcinoma tissue, human skin tissue, human breast carcinoma tissue, mouse colon tissue, Jurkat.
Subcellular location: Nucleus. Cytoplasm.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  FC
1:500-1:2,000
1:50-1:100
1:100-1:500
1:50-1:100
Uniprot #: SwissProt: P09651 Human | P49312 Mouse | P04256 Rat
Alternative names: HNRNPA 1 Helix destabilizing protein Helix-destabilizing protein Heterogeneous nuclear ribonucleoprotein A1 Heterogeneous nuclear ribonucleoprotein A1B protein Heterogeneous nuclear ribonucleoprotein B2 protein Heterogeneous nuclear ribonucleoprotein core protein A1 hnRNP A1 hnRNP core protein A1 HNRNPA1 HNRPA1 MGC102835 Nuclear ribonucleoprotein particle A1 protein ROA1_HUMAN Single strand DNA binding protein UP1 Single strand RNA binding protein Single-strand RNA-binding protein
Images
ET1704-52_1.jpg Fig1: Western blot analysis of hnRNP A1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1704-52, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: HepG2 cell lysate
Lane 2: PC-12 cell lysate
ET1704-52_2.jpg Fig2: ICC staining of hnRNP A1 in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1704-52, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1704-52_3.jpg Fig3: ICC staining of hnRNP A1 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1704-52, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1704-52_4.jpg Fig4: ICC staining of hnRNP A1 in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1704-52, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1704-52_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-hnRNP A1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-52, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1704-52_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-hnRNP A1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-52, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1704-52_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-hnRNP A1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-52, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1704-52_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-hnRNP A1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-52, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1704-52_9.jpg Fig9: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-hnRNP A1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-52, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1704-52_10.jpg Fig10: Flow cytometric analysis of hnRNP A1 was done on Jurkat cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1704-52, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
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