CHD4 Recombinant Rabbit Monoclonal Antibody [JA33-40]
cat.: ET1704-53
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JA33-40 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 218 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human CHD4 aa 1,811-1,860. |
| Positive control: | 293T cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, 293T, human breast carcinoma tissue, human colon carcinoma tissue. |
| Subcellular location: | Nucleus. Cytoplasm. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500-1:2,000 1:50-1:200 1:50-1:400 1:50-1:100 |
| Uniprot #: | SwissProt: Q14839 Human | Q6PDQ2 Mouse |
| Alternative names: | ATP dependent helicase CHD4 ATP-dependent helicase CHD4 CHD 4 CHD-4 CHD4 CHD4_HUMAN Chromodomain helicase DNA binding protein 4 Chromodomain-helicase-DNA-binding protein 4 Mi 2 autoantigen 218 kDa protein Mi 2b Mi-2 autoantigen 218 kDa protein Mi2 beta Mi2-beta |
Images
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Fig1:
Western blot analysis of CHD4 on different lysates with Rabbit anti-CHD4 antibody (ET1704-53) at 1/500 dilution. Lane 1: 293T cell lysate Lane 2: NIH/3T3 cell lysate Lane 3: C2C12 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 218 kDa Observed band size: 260 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-53) at 1/500 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2: ICC staining of CHD4 in 293T cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1704-53, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue). |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-CHD4 antibody (ET1704-53) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-53) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Flow cytometric analysis of CHD4 was done on 293T cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1704-53, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig5:
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-CHD4 antibody (ET1704-53) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-53) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.