ET1704-54

EAAT1 Recombinant Rabbit Monoclonal Antibody [JA30-35]

cat.: ET1704-54

Product Type:	Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity:	Human, Mouse, Rat, Cynomolgus monkey, Pig
Applications:	WB, IHC-P, IF-Tissue, IHC-Fr, mIHC
Clonality:	Monoclonal
Clone number:	JA30-35

Form:	Liquid
Storage condition:	Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage buffer:	1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Concentration:	1ug/ul
Purification:	Protein A affinity purified.
Molecular weight:	Predicted band size: 60 kDa
Isotype:	IgG
Immunogen:	Synthetic peptide within Human EAAT1 aa 171-220 / 542.
Positive control:	Human cerebellum tissue, mouse cerebellum tissue, rat cerebellum tissue, Mouse brain tissue lysate, Mouse cerebellum tissue lysate, Rat brain tissue lysate, Rat cerebellum tissue lysate.
Subcellular location:	Cell membrane.
Recommended Dilutions: WB IHC-P IF-Tissue IHC-Fr mIHC	1:5,000 1:1,000-1:8,000 1:500 1:500 1:5,000
Uniprot #:	SwissProt: P43003 Human \| P56564 Mouse \| P24942 Rat
Alternative names:	GLAST-1 SLC1A3 EAAT1 GLAST GLAST1

Images

	Fig1: mIHC analysis of mouse cerebellum tissue (paraffin-embedded) with EAAT1 (ET1704-54, 1/5,000), NeuN (HA601482, 1:10,000) and MBP (ET1702-15, 1/10,000). The immunostaining was performed with the IRISKitCmTSA Kit (900802). Blocking with 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
	Fig2: Application: IHC-Fr Species: Mouse Site: Cerebellum Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: Not required
	Fig3: Application: IHC-Fr Species: Rat Site: Cerebellum Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: Not required
	Fig4: Application: IF-tissue Species: Mouse Site: Cerebellum Sample: Paraffin-embedded section Antibody concentration: 1:500

	Fig5: Application: IF-tissue Species: Rat Site: Cerebellum Sample: Paraffin-embedded section Antibody concentration: 1:500
	Fig6: Immunohistochemical analysis of paraffin-embedded human cerebellum tissue with Rabbit anti-EAAT1 antibody (ET1704-54) at 1/8,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1704-54) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig7: Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-EAAT1 antibody (ET1704-54) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1704-54) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Fig8: Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-EAAT1 antibody (ET1704-54) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1704-54) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Fig9: Western blot analysis of EAAT1 on different lysates with Rabbit anti-EAAT1 antibody (ET1704-54) at 1/5,000 dilution.

Lane 1: Mouse brain tissue lysate (no heat)
Lane 2: Mouse cerebellum tissue lysate
Lane 3: Mouse brain tissue lysate treated with deglycosylation
Lane 4: Mouse brain tissue lysate
Lane 5: Rat brain tissue lysate (no heat)
Lane 6: Rat cerebellum tissue lysate

Notice: no heat means the lysate is not boiled.

Lysates/proteins at 10 µg/Lane.

Predicted band size: 60 kDa
Observed band size: 60-250 kDa

Exposure time: 25 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-54) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.