MRP1 Recombinant Rabbit Monoclonal Antibody [JA93-03]
cat.: ET1704-56
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, IHC-P, FC, IF-Cell
Clonality: Monoclonal
Clone number: JA93-03
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 172 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human MRP1 aa 1482-1531 / 1531.
Positive control: A549 cell lysate, HepG2 cell lysate, A549, human pancreas tissue, human tonsil tissue.
Subcellular location: Cell membrane.
Recommended Dilutions:
  WB
  IHC-P
  FC
  IF-Cell
1:1,000
1:50-1:200
1:1,000
1:100
Uniprot #: SwissProt: P33527 Human
Alternative names: ABC 29 ABC29 ABCC 1 ABCC Abcc1 ATP binding cassette sub family C (CFTR/MRP) member 1 ATP binding cassette sub-family C member 1 ATP binding cassette subfamily C member 1 ATP binding cassette transporter variant ABCC1delta ex13 ATP binding cassette transporter variant ABCC1delta ex13&14 ATP binding cassette transporter variant ABCC1delta ex25 ATP binding cassette transporter variant ABCC1delta ex25&26 ATP binding cassette, sub-family C (CFTR/MRP), member 1 ATP-binding cassette sub-family C member 1 DKFZp686N04233 DKFZp781G125 GS X GSX Leukotriene C(4) transporter LTC4 transporter MRP 1 MRP MRP1 MRP1_HUMAN Multidrug resistance associated protein 1 Multidrug resistance protein Multidrug resistance-associated protein 1 Multiple drug resistance associated protein Multiple drug resistance protein 1
Images
ET1704-56_1.jpg Fig1: Western blot analysis of MRP1 on different lysates with Rabbit anti-MRP1 antibody (ET1704-56) at 1/1,000 dilution.

Lane 1: A549 cell lysate
Lane 2: A549 cell lysate (no heat)
Lane 3: HepG2 cell lysate
Lane 4: HepG2 cell lysate (no heat)

Lysates/proteins at 20 µg/Lane.

Predicted band size: 172 kDa
Observed band size: 220 kDa

Exposure time: 3 minutes; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-56) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET1704-56_2.jpg Fig2: Western blot analysis of MRP1 on different lysates with Rabbit anti-MRP1 antibody (ET1704-56) at 1/1,000 dilution.

Lane 1: A549-si NT cell lysate (no heat)
Lane 2: A549-si MRP1 cell lysate (no heat)

Lysates/proteins at 10 µg/Lane.

Predicted band size: 172 kDa
Observed band size: 220 kDa

Exposure time: 3 minutes; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-56) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET1704-56_3.jpg Fig3: Immunocytochemistry analysis of A549 cells labeling MRP1 with Rabbit anti-MRP1 antibody (ET1704-56) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-MRP1 antibody (ET1704-56) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
ET1704-56_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-MRP1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-56, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1704-56_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-MRP1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-56, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1704-56_6.jpg Fig6: Flow cytometric analysis of A549 cells labeling MRP1.

Cells were fixed and permeabilized. Then stained with the primary antibody (ET1704-56, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.