ET1704-59

Glucose Transporter GLUT3 Recombinant Rabbit Monoclonal Antibody [JA50-31]

cat.: ET1704-59

Product Type:	Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity:	Human, Mouse, Rat
Applications:	WB, IHC-P, FC
Clonality:	Monoclonal
Clone number:	JA50-31

Form:	Liquid
Storage condition:	Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer:	1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration:	1ug/ul
Purification:	Protein A affinity purified.
Molecular weight:	Predicted band size: 54 kDa
Isotype:	IgG
Immunogen:	Synthetic peptide within Human GLUT3 aa 11-60 / 496.
Positive control:	Rat heart tissue lysate, mouse heart tissue lysate, HepG2 cell lysate, human brain tissue, human embryo tissue, mouse brain tissue, SH-SY5Y.
Subcellular location:	Cell membrane. Perikaryon. Cell projection.
Recommended Dilutions: WB IHC-P FC	1:500-1:2,000 1:50-1:200 1:50-1:100
Uniprot #:	SwissProt: P11169 Human \| P32037 Mouse \| Q07647 Rat
Alternative names:	brain FLJ90380 Glucose transporter type 3 Glucose transporter type 3 brain GLUT 3 GLUT-3 GLUT3 GTR3_HUMAN Slc2a3 Solute Carrier Family 2 (Facilitated Glucose Transporter) Member 3 Solute carrier family 2, facilitated glucose transporter member 3

Images

	Fig1: Western blot analysis of Glucose Transporter GLUT3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1704-59, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: rat heart tissue lysate Lane 2: mouse heart tissue lysate Lane 3: HepG2 cell lysate
	Fig2: Western blot analysis of Glucose Transporter GLUT3 on different lysates with Rabbit anti-Glucose Transporter GLUT3 antibody (ET1704-59) at 1/1,000 dilution. Lane 1: HepG2-si NT cell lysate Lane 2: HepG2-si Glucose Transporter GLUT3 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 54 kDa Observed band size: 54 kDa Exposure time: 1 minute; ECL: merk 4-20% SDS-PAGE gel. ET1704-59 was shown to specifically react with Glucose Transporter GLUT3 in HepG2-si NT cells. Weakened band was observed when HepG2-si Glucose Transporter GLUT3 sample was tested. HepG2-si NT and HepG2-si Glucose Transporter GLUT3 samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1704-59, 1/1,000) and Loading control antibody (Rabbit anti-GAPDH, ET1601-4, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
	Fig3: Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-Glucose Transporter GLUT3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1704-59, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig4: Immunohistochemical analysis of paraffin-embedded human embryo tissue using anti-Glucose Transporter GLUT3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1704-59, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig5: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Glucose Transporter GLUT3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1704-59, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig6: Flow cytometric analysis of Glucose Transporter GLUT3 was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1704-59, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.