Alpha B Crystallin Recombinant Rabbit Monoclonal Antibody [JA50-32]
cat.: ET1704-60
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB, IHC-P, IF-Cell, IF-Tissue, IP |
| Clonality: | Monoclonal |
| Clone number: | JA50-32 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 20 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Alpha B Crystallin aa 126-175 / 175. |
| Positive control: | Rat heart tissue lysate, human skeletal muscle tissue lysate, Hela, human embryonic skeletal muscle tissue, human prostate tissue. |
| Subcellular location: | Nucleus, Cytoplasm. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue IHC-P IP |
1:500-1:2,000 1:50-1:200 1:50-1:200 1:50-1:200 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P02511 Human | P23928 Rat |
| Alternative names: | AACRYA Alpha B crystallin Alpha crystallin B chain Alpha(B)-crystallin Alpha-crystallin B chain CRYA2 Cryab CRYAB_HUMAN Crystallin alpha B Crystallin alpha polypeptide 2 CTPP2 Heat shock 20 kD like protein Heat shock protein beta 5 Heat shock protein beta-5 HspB5 Renal carcinoma antigen NY REN 27 Renal carcinoma antigen NY-REN-27 Rosenthal fiber component |
Images
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Fig1:
Western blot analysis of Alpha B Crystallin on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1704-60, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:20,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Rat heart tissue lysate Lane 2: Human skeletal muscle tissue lysate |
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Fig2: ICC staining of Alpha B Crystallin in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1704-60, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig3: Immunohistochemical analysis of paraffin-embedded human embryonic skeletal muscle tissue using anti-Alpha B Crystallin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-60, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-Alpha B Crystallin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-60, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.