Periostin Recombinant Rabbit Monoclonal Antibody [JA63-40]
cat.: ET1704-65
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JA63-40 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 93 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic pepride within Human Perilipin-1 aa 11-60 / 836. |
| Positive control: | HaCaT cell lysate, HFF-1 cell lysate, BxPC-3 cell lysate, SH-SY5Y cell lysate, 293T cell lysate, human skin tissue lysate, human lung tissue lysate, human brain tissue lysate, human skin tissue. |
| Subcellular location: | Golgi apparatus, Secreted, extracellular space, extracellular matrix. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:400 |
| Uniprot #: | SwissProt: Q15063 Human |
| Alternative names: | Fasciclin-I like MGC119510 MGC119511 OSF 2 OSF-2 OSF2 Osteoblast specific factor 2 (fasciclin I like) Osteoblast specific factor 2 Osteoblast specific factor Osteoblast-specific factor 2 PDLPOSTN Periodontal ligament specific periostin Periostin Periostin isoform thy2 Periostin isoform thy4 Periostin isoform thy6 Periostin isoform thy8 Periostin osteoblast specific factor PN POSTN POSTN_HUMAN RP11 412K4.1 |
Images
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Fig1:
Western blot analysis of Periostin on different lysates with Rabbit anti-Periostin antibody (ET1704-65) at 1/1,000 dilution. Lane 1: HaCaT cell lysate Lane 2: HFF-1 cell lysate Lane 3: BxPC-3 cell lysate Lane 4: SH-SY5Y cell lysate Lane 5: 293T cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 93 kDa Observed band size: 85 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-65) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of Periostin on different lysates with Rabbit anti-Periostin antibody (ET1704-65) at 1/1,000 dilution. Lane 1: Human skin tissue lysate Lane 2: Human lung tissue lysate Lane 3: Human brain tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 93 kDa Observed band size: 85 kDa Exposure time: Lane 1: 3 minutes; Lane 2-3: 24 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-65) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature. |
|
Fig3: Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-Periostin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-65, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.