Periostin Recombinant Rabbit Monoclonal Antibody [JA63-40]
cat.: ET1704-65
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JA63-40 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 93 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic pepride within Human Perilipin-1 aa 11-60 / 836. |
| Positive control: | Human skin tissue lysates, 293T, human skin tissue, human lung tissue lysate, human brain tissue lysate. |
| Subcellular location: | Golgi apparatus, Secreted, extracellular space, extracellular matrix. |
| Recommended Dilutions:
WB IHC-P FC |
1:1,000 1:400 1:50 |
| Uniprot #: | SwissProt: Q15063 Human |
| Alternative names: | Fasciclin-I like MGC119510 MGC119511 OSF 2 OSF-2 OSF2 Osteoblast specific factor 2 (fasciclin I like) Osteoblast specific factor 2 Osteoblast specific factor Osteoblast-specific factor 2 PDLPOSTN Periodontal ligament specific periostin Periostin Periostin isoform thy2 Periostin isoform thy4 Periostin isoform thy6 Periostin isoform thy8 Periostin osteoblast specific factor PN POSTN POSTN_HUMAN RP11 412K4.1 |
Images
|
Fig1:
Western blot analysis of Periostin on human skin tissue lysates with Rabbit anti-Periostin antibody (ET1704-65) at 1/1,000 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 93 kDa Observed band size: 85 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-65) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig2: Flow cytometric analysis of Periostin was done on 293T cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1704-65, 1/50) (blue). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; red). |
|
Fig3: Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-Periostin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-65, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Western blot analysis of Periostin on different lysates with Rabbit anti-Periostin antibody (ET1704-65) at 1/1,000 dilution. Lane 1: Human lung tissue lysate Lane 2: Human brain tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 93 kDa Observed band size: 85 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-65) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.