CDT1 Recombinant Rabbit Monoclonal Antibody [JA30-81]
cat.: ET1704-67
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, IHC-P, IP
Clonality: Monoclonal
Clone number: JA30-81
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 60 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human CDT1 aa 497-546 / 546.
Positive control: Hela cell lysate, 293 cell lysate, human skin tissue.
Subcellular location: Nucleus.
Recommended Dilutions:
  WB
  IHC-P

1:500-1:1,000
1:25-1:50
Uniprot #: SwissProt: Q9H211 Human
Alternative names: CDT 1 cdt1 CDT1_HUMAN Chromatin licensing and DNA replication factor 1 DNA replication factor DNA replication factor Cdt1 Double parked Double parked Drosophila homolog of Double parked homolog DUP Retroviral integration site 1 Retroviral integration site 2 Retroviral integration site1 Retroviral integration site2 RIS 2 RIS2
Images
ET1704-67_1.jpg Fig1: Western blot analysis of CDT1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1704-67, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Hela cell lysate
Lane 2: 293 cell lysate
ET1704-67_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-CDT1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-67, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.