CDT1 Recombinant Rabbit Monoclonal Antibody [JA30-81]
cat.: ET1704-67
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | JA30-81 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 60 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human CDT1 aa 497-546 / 546. |
| Positive control: | HCT 116 cell lysate, HT-29 cell lysate, Jurkat cell lysate, HEK-293 cell lysate, HT-29, human skin tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IHC-P IF-Cell |
1:2,000 1:25-1:50 1:100 |
| Uniprot #: | SwissProt: Q9H211 Human |
| Alternative names: | CDT 1 cdt1 CDT1_HUMAN Chromatin licensing and DNA replication factor 1 DNA replication factor DNA replication factor Cdt1 Double parked Double parked Drosophila homolog of Double parked homolog DUP Retroviral integration site 1 Retroviral integration site 2 Retroviral integration site1 Retroviral integration site2 RIS 2 RIS2 |
Images
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Fig1:
Western blot analysis of CDT1 on different lysates with Rabbit anti-CDT1 antibody (ET1704-67) at 1/2,000 dilution. Lane 1: HCT 116 cell lysate Lane 2: HT-29 cell lysate Lane 3: Jurkat cell lysate Lane 4: HEK-293 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 60 kDa Observed band size: 70 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-67) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunocytochemistry analysis of HT-29 cells labeling CDT1 with Rabbit anti-CDT1 antibody (ET1704-67) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CDT1 antibody (ET1704-67) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-CDT1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-67, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.