PDCD4 Recombinant Rabbit Monoclonal Antibody [JA80-83]
cat.: ET1704-70
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IHC-P, FC
Clonality: Monoclonal
Clone number: JA80-83
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 52 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human PDCD4 aa 425-469/469.
Positive control: Rat pancreas tissue lysate, Daudi cell lysate, A431, human tonsil tissue, mouse colon tissue, MCF-7.
Subcellular location: Nucleus. Cytoplasm.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  FC

1:500
1:100
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: Q53EL6 Human | Q61823 Mouse | Q9JID1 Rat
Alternative names: Death up-regulated gene protein Dug H731 Ma3 MGC33046 MGC33047 Neoplastic transformation inhibitor Neoplastic transformation inhibitor protein Nuclear antigen H731 Nuclear antigen H731 like Nuclear antigen H731 like protein Nuclear antigen H731-like PDCD 4 Pdcd4 PDCD4_HUMAN Programmed cell death 4 programmed cell death 4 (neoplastic transformation inhibitor) Programmed cell death protein 4 Protein 197/15a Protein MA-3 Tis Topoisomerase-inhibitor suppressed protein
Images
ET1704-70_1.jpg Fig1: Western blot analysis of PDCD4 on different lysates with Rabbit anti-PDCD4 antibody (ET1704-70) at 1/500 dilution.

Lane 1: Rat pancreas tissue lysate (20 µg/Lane)
Lane 2: Daudi cell lysate (10 µg/Lane)

Predicted band size: 52 kDa
Observed band size: 65 kDa

Exposure time: 1 minute;

10% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-70) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
ET1704-70_2.jpg Fig2: Immunocytochemistry analysis of A431 cells labeling PDCD4 with Rabbit anti-PDCD4 antibody (ET1704-70) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PDCD4 antibody (ET1704-70) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
ET1704-70_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-PDCD4 antibody (ET1704-70) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-70) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1704-70_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-PDCD4 antibody (ET1704-70) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-70) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1704-70_5.jpg Fig5: Flow cytometric analysis of MCF-7 cells with PDCD4 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.