CD39 Recombinant Rabbit Monoclonal Antibody [JA90-36]
cat.: ET1704-74
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P
Clonality: Monoclonal
Clone number: JA90-36
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 58 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human CD39 aa 371-420 / 510.
Positive control: Mouse lung tissue lysate, mouse placenta tissue lysate, human tonsil tissue, human spleen tissue, human placenta tissue, mouse uterus tissue, human endometrium tissue.
Subcellular location: Membrane.
Recommended Dilutions:
  WB
  IHC-P
1:5,000
1:50-1:400
Uniprot #: SwissProt: P49961 Human | P55772 Mouse | P97687 Rat
Alternative names: ATPDase CD 39 CD39 CD39 antigen DKFZp686D194 DKFZp686I093 Ecto apyrase Ecto ATP diphosphohydrolase Ecto-apyrase Ecto-ATP diphosphohydrolase 1 Ecto-ATPase 1 Ecto-ATPDase 1 Ectonucleoside triphosphate diphosphohydrolase 1 ENTP1_HUMAN ENTPD 1 ENTPD1 FLJ40921 FLJ40959 Lymphoid cell activation antigen NTPDase 1 NTPDase1 SPG64
Images
ET1704-74_1.jpg Fig1: Western blot analysis of CD39 on different lysates with Rabbit anti-CD39 antibody (ET1704-74) at 1/5,000 dilution.

Lane 1: Jurkat cell lysate (negative) (15 µg/Lane)
Lane 2: Mouse lung tissue lysate (20 µg/Lane)
Lane 3: Mouse placenta tissue lysate (20 µg/Lane)

Predicted band size: 58 kDa
Observed band size: 70 kDa

Exposure time: 20 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-74) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET1704-74_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD39 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-74, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1704-74_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD39 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-74, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1704-74_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-CD39 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-74, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1704-74_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse uterus tissue using anti-CD39 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-74, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1704-74_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human endometrium tissue with Rabbit anti-CD39 antibody (ET1704-74) at 1/400 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-74) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.