DAZL Recombinant Rabbit Monoclonal Antibody [JA10-36]
cat.: ET1704-75
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JA10-36 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 33 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within DAZL aa 10-59 / 295. |
| Positive control: | Human testis tissue, rat testis tissue. |
| Subcellular location: | Cytoplasm. Nucleus. Predominantly cytoplasmic. |
| Recommended Dilutions:
WB IHC-P |
1:500 1:400 |
| Uniprot #: | SwissProt: Q92904 Human | Q64368 Mouse Entrez Gene: 680486 Rat |
| Alternative names: | DAZ homolog DAZ like autosomal DAZ-like autosomal DAZH DAZL 1 DAZL DAZL_HUMAN DAZL1 DAZLA Deleted in azoospermia like 1 Deleted in azoospermia like Deleted in azoospermia like autosomal Deleted in azoospermia-like 1 Deleted in azoospermia-like Germline specific RNA binding protein MGC26406 Spermatogenesis gene on the Y like autosomal SPGY like autosomal SPGY-like-autosomal SPGYLA Tpx2 |
Images
|
Fig1:
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-DAZL antibody (ET1704-75) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-75) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-DAZL antibody (ET1704-75) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-75) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.